Plantations of native shrub species restore soil microbial diversity in the Horqin Sandy Land, northeastern China
DeMing JIANG, ChengYou CAO, Ying ZHANG, ZhenBo CUI, XiaoShu HAN
1 Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2 College of Life and Health Sciences, Northeastern University, Shenyang 110004, China
Plantations of native shrub species restore soil microbial diversity in the Horqin Sandy Land, northeastern China
DeMing JIANG, ChengYou CAO, Ying ZHANG, ZhenBo CUI, XiaoShu HAN
1 Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2 College of Life and Health Sciences, Northeastern University, Shenyang 110004, China
摘要 Caragana microphylla Lam., a leguminous shrub species, plays an important role in revegetation in the degraded ecosystems of the Horqin Sandy Land, northeastern China. Large areas planted with this shrub have been artificially established as sand binders for soil protection, which might change the composition of soil bacterial communities with the development of sand dune stabilization. In this paper, we investigated the diversity and composition of native soil bacterial communities in the C. microphylla plantation for sand fixation using polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) to understand the influence of this plantation on sandy soil ecosystem development. We collected soil samples from plantations with an age sequence of 0, 9, 16, and 26 years, as well as from the natural community, to identify the differences among soil bacterial communities. The result showed that bacterial abundance and community composition in the sandy land were affected by the age of the C. microphylla plantation. Moreover, bacterial diversity decreased with increasing plantation age, and the composition of the bacterial community in the 26-year plantation was similar to that in the natural community. Phylogenetic analysis of bands excised from the DGGE gels showed that members of alpha Proteobacterium,
gamma Proteobacterium, Gemmatimonadetes and Chloroflexi were dominant in the sandy land. The stabilization of moving sand dune and development of sand-fixed plantation resulted in an increase of soil fertility, which could drive the structural evolvement of soil bacterial community, and it needs over 20 years for the soil bacterial community to form a stable structure, similar to the case for the natural vegetation.
Abstract: Caragana microphylla Lam., a leguminous shrub species, plays an important role in revegetation in the degraded ecosystems of the Horqin Sandy Land, northeastern China. Large areas planted with this shrub have been artificially established as sand binders for soil protection, which might change the composition of soil bacterial communities with the development of sand dune stabilization. In this paper, we investigated the diversity and composition of native soil bacterial communities in the C. microphylla plantation for sand fixation using polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) to understand the influence of this plantation on sandy soil ecosystem development. We collected soil samples from plantations with an age sequence of 0, 9, 16, and 26 years, as well as from the natural community, to identify the differences among soil bacterial communities. The result showed that bacterial abundance and community composition in the sandy land were affected by the age of the C. microphylla plantation. Moreover, bacterial diversity decreased with increasing plantation age, and the composition of the bacterial community in the 26-year plantation was similar to that in the natural community. Phylogenetic analysis of bands excised from the DGGE gels showed that members of alpha Proteobacterium,
gamma Proteobacterium, Gemmatimonadetes and Chloroflexi were dominant in the sandy land. The stabilization of moving sand dune and development of sand-fixed plantation resulted in an increase of soil fertility, which could drive the structural evolvement of soil bacterial community, and it needs over 20 years for the soil bacterial community to form a stable structure, similar to the case for the natural vegetation.
This work was supported by the National Natural Science Foundation of China (40871247) and the China National Twelfth Five-year-plan Key Project (2012BAD16B0302). The authors thank the members of the Wulanaodu Station of Desertification Research, Chinese Academy of Sciences for their technical assistance.
通讯作者:
ChengYou CAO
E-mail: caochengyou@163.com
引用本文:
DeMing JIANG, ChengYou CAO, Ying ZHANG, ZhenBo CUI, XiaoShu HAN. Plantations of native shrub species restore soil microbial diversity in the Horqin Sandy Land, northeastern China[J]. 干旱区科学, 2014, 6(4): 445-453.
DeMing JIANG, ChengYou CAO, Ying ZHANG, ZhenBo CUI, XiaoShu HAN. Plantations of native shrub species restore soil microbial diversity in the Horqin Sandy Land, northeastern China. Journal of Arid Land, 2014, 6(4): 445-453.
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2014, Vol. 6 
