Microbial diversity in the saline-alkali soil of a coastal Tamarix chinensis woodland at Bohai Bay, China
LIU Wanqiu1,2,3, ZHANG Wei1,2, LIU Guangxiu1,2*, ZHANG Yanhua3, ZHANG Gaosen1,2
1 Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China;
2 Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou 730000, China;
3 Ocean and Fishery Bureau of Changyi, Changyi 261300, China
Microbial diversity in the saline-alkali soil of a coastal Tamarix chinensis woodland at Bohai Bay, China
LIU Wanqiu1,2,3, ZHANG Wei1,2, LIU Guangxiu1,2*, ZHANG Yanhua3, ZHANG Gaosen1,2
1 Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China;
2 Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou 730000, China;
3 Ocean and Fishery Bureau of Changyi, Changyi 261300, China
摘要 Soil salinization or alkalization is a form of soil desertification. Coastal saline-alkali soil represents a type of desert and a key system in the network of ecosystems at the continent-ocean interface. Tamarix chinensis is a drought-tolerant plant that is widely distributed in the coastal saline-alkali soil of Bohai Bay, China. In this study, we used 454 pyrosequencing techniques to investigate the characteristics and distribution of the microbial diversity in coastal saline-alkali soil of the T. chinensis woodland at Bohai Bay. A total of 20,315 sequences were obtained, representing 19 known bacterial phyla and a large proportion of unclassified bacteria at the phylum level. Proteobacteria, Acidobacteria and Actinobacteria were the predominant phyla. The coverage of T. chinensis affected the microbial composition. At the phylum level, the relative abundance of γ-Proteobacteria and Bacteroidetes decreased whereas Actinobacteria increased with the increasing coverage of T. chinensis. At the genus level, the proportions of Steroidobacter, Lechevalieria, Gp3 and Gp4 decreased with the increase of the vegetation coverage whereas the proportion of Nocardioides increased. A cluster analysis showed that the existing T. chinensis changed the niches for the microorganisms in the coastal saline-alkali soil, which caused changes in the microbial community. The analysis also distinguished the microbial community structure of the marginal area from those of the dense area and sparse area. Furthermore, the results also indicated that the distance to the seashore line could also affect certain groups of soil bacteria in this coastal saline-alkali soil, such as the family Cryomorphaceae and class Flavobacteria, whose population decreased as the distance increased. In addition, the seawater and temperature could be the driving factors that affected the changes.
Abstract: Soil salinization or alkalization is a form of soil desertification. Coastal saline-alkali soil represents a type of desert and a key system in the network of ecosystems at the continent-ocean interface. Tamarix chinensis is a drought-tolerant plant that is widely distributed in the coastal saline-alkali soil of Bohai Bay, China. In this study, we used 454 pyrosequencing techniques to investigate the characteristics and distribution of the microbial diversity in coastal saline-alkali soil of the T. chinensis woodland at Bohai Bay. A total of 20,315 sequences were obtained, representing 19 known bacterial phyla and a large proportion of unclassified bacteria at the phylum level. Proteobacteria, Acidobacteria and Actinobacteria were the predominant phyla. The coverage of T. chinensis affected the microbial composition. At the phylum level, the relative abundance of γ-Proteobacteria and Bacteroidetes decreased whereas Actinobacteria increased with the increasing coverage of T. chinensis. At the genus level, the proportions of Steroidobacter, Lechevalieria, Gp3 and Gp4 decreased with the increase of the vegetation coverage whereas the proportion of Nocardioides increased. A cluster analysis showed that the existing T. chinensis changed the niches for the microorganisms in the coastal saline-alkali soil, which caused changes in the microbial community. The analysis also distinguished the microbial community structure of the marginal area from those of the dense area and sparse area. Furthermore, the results also indicated that the distance to the seashore line could also affect certain groups of soil bacteria in this coastal saline-alkali soil, such as the family Cryomorphaceae and class Flavobacteria, whose population decreased as the distance increased. In addition, the seawater and temperature could be the driving factors that affected the changes.
This study was funded by the National Natural Science Foundation of China (31470544, 41271265), a special financial grant from the China Postdoctoral Science Foundation (2013T60900), and the Science and Technology Projects in Gansu Province (1304NKCA135).
通讯作者:
LIU Guangxiu
E-mail: liugx@lzb.ac.cn
引用本文:
LIU Wanqiu, ZHANG Wei, LIU Guangxiu, ZHANG Yanhua, ZHANG Gaosen. Microbial diversity in the saline-alkali soil of a coastal Tamarix chinensis woodland at Bohai Bay, China[J]. 干旱区科学, 2016, 8(2): 284-292.
LIU Wanqiu, ZHANG Wei, LIU Guangxiu, ZHANG Yanhua, ZHANG Gaosen. Microbial diversity in the saline-alkali soil of a coastal Tamarix chinensis woodland at Bohai Bay, China. Journal of Arid Land, 2016, 8(2): 284-292.
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2016, Vol. 8 
