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Journal of Arid Land  2024, Vol. 16 Issue (3): 415-430    DOI: 10.1007/s40333-024-0007-1     CSTR: 32276.14.s40333-024-0007-1
Research article     
Effects of land-use patterns on soil microbial diversity and composition in the Loess Plateau, China
ZHANG Jian, GUO Xiaoqun, SHAN Yujie, LU Xin, CAO Jianjun*()
College of Geography and Environmental Science, Northwest Normal University, Lanzhou 730070, China
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Abstract  

In the Loess Plateau of China, land-use pattern is a major factor in controlling underlying biological processes. Additionally, the process of land-use pattern was accompanied by abandoned lands, potentially impacting soil microbe. However, limited researches were conducted to study the impacts of land-use patterns on the diversity and community of soil microorganisms in this area. The study aimed to investigate soil microbial community diversity and composition using high-throughput deoxyribonucleic acid (DNA) sequencing under different land-use patterns (apricot tree land, apple tree land, peach tree land, corn land, and abandoned land). The results showed a substantial difference (P<0.050) in bacterial alpha-diversity and beta-diversity between abandoned land and other land-use patterns, with the exception of Shannon index. While fungal beta-diversity was not considerably impacted by land-use patterns, fungal alpha-diversity indices varied significantly. The relative abundance of Actinobacteriota (34.90%), Proteobacteria (20.65%), and Ascomycota (77.42%) varied in soils with different land-use patterns. Soil pH exerted a dominant impact on the soil bacterial communities' composition, whereas soil available phosphorus was the main factor shaping the soil fungal communities' composition. These findings suggest that variations in land-use pattern had resulted in changes to soil properties, subsequently impacting diversity and structure of microbial community in the Loess Plateau. Given the strong interdependence between soil and its microbiota, it is imperative to reclaim abandoned lands to maintain soil fertility and sustain its function, which will have significant ecological service implications, particularly with regards to soil conservation in ecologically vulnerable areas.



Key wordsabandoned lands      land-use pattern      soil property      diversity of soil microbe      soil microbial community     
Received: 19 September 2023      Published: 31 March 2024
Corresponding Authors: *CAO Jianjun (E-mail: caojj@nwnu.edu.cn)
Cite this article:

ZHANG Jian, GUO Xiaoqun, SHAN Yujie, LU Xin, CAO Jianjun. Effects of land-use patterns on soil microbial diversity and composition in the Loess Plateau, China. Journal of Arid Land, 2024, 16(3): 415-430.

URL:

http://jal.xjegi.com/10.1007/s40333-024-0007-1     OR     http://jal.xjegi.com/Y2024/V16/I3/415

Fig. 1 (a), location of the sampling sites; (b), AL (abandoned land); (c), AT (apple land); (d), ATL (apricot land); (e), CL (corn land); (f), PTL (peach land). The abbreviations are the same as in the following figures. Note that the figure 1a is based on the standard map of the Earth Online (https://www.earthol.com/bd/), and the standard map has not been modified.
Soil property AT PTL ATL CL AL
SOC (g/kg) 7.13±0.60a 7.58±1.57a 5.67±0.35a 7.34±1.10a 6.48±1.53a
TN (g/kg) 0.24±0.14b 0.56±0.10a 0.25±0.04b 0.36±0.04ab 0.18±0.04b
TP (g/kg) 0.63±0.13ab 0.31±0.08b 0.99±0.41a 0.19±0.07b 0.40±0.07b
NH4+-N (mg/kg) 2.81±0.37ab 2.69±0.44ab 4.16±0.88a 3.26±0.50ab 2.27±0.38b
NO3--N (mg/kg) 28.24±3.18ab 23.43±4.52bc 11.06±1.24d 33.76±2.76a 18.54±2.88cd
AP (mg/kg) 9.37±0.93bc 11.79±1.70ab 14.06±1.00a 8.97±1.27bc 7.03±0.63c
pH 8.47±0.04a 8.45±0.03ab 8.28±0.12b 8.41±0.03ab 8.55±0.03a
SS (g/kg) 0.06±0.002a 0.06±0.003a 0.09±0.03a 0.07±0.01a 0.05±0.01a
SWC (%) 0.48±0.32a 0.16±0.01a 0.13±0.01a 0.19±0.01a 0.15±0.04a
BD (g/cm3) 1.73±0.03a 1.67±0.01ab 1.72±0.06a 1.65±0.03ab 1.57±0.08b
Table 1 Soil properties among different land-use patterns
Microbe Land-use pattern Good's coverage (%) Chao1 Shannon Sobs
Bacteria AT 0.9612b 4091.90±58.99a 6.6763±0.02a 2857.80±43.07a
PTL 0.9623b 3982.55±137.66a 6.6276±0.05a 2852.20±81.26a
ATL 0.9626b 3906.05±100.82a 6.5571±0.07a 2755.40±83.67a
CL 0.9636b 3898.12±94.33a 6.6394±0.01a 2753.00±34.95a
AL 0.9691a 3311.57±257.73b 6.4621±0.14a 2427.60±170.74b
Fungi AT 0.9979b 500.38±14.10a 4.3488±0.13a 442.80±15.69a
PTL 0.9988a 371.67±30.87b 3.8334±0.28ab 349.40±28.77b
ATL 0.9980b 415.26±26.32b 3.4909±0.31b 365.80±26.67b
CL 0.9979b 414.17±28.91b 3.5820±0.41ab 355.20±28.91b
AL 0.9988a 365.56±28.63b 3.9021±0.23ab 336.20±25.52b
Table 2 Differences in microbial diversity among different land-use patterns
Fig. 2 Non-metric multidimensional scaling (NMDS) analysis of bacteria (a) and fungi (b) among different land-use patterns at the phylum level. R value is the ANOSIM (analysis of similarities) statistic R, and P value is the significance from permutation.
Fig. 3 Relative abundance of dominant bacterial (a and c) and fungal (b and d) communities at the phylum level among different land-use patterns. Different lowercase letters within the same microbe indicate significant differences under different land-use patterns at P<0.050 level.
Fig. 4 Venn diagram represents the operational taxonomic units (OUTs) of bacterial (a) and fungal (b) communities among different land-use patterns
Fig. 5 Cladogram depicting the phylogenetic distribution of bacterial (a) and fungal (b) lineages among different land-use patterns. Linear discriminant analysis (LDA) score histogram was computed for species with varying abundances in bacterial and fungal communities, and identified using a threshold value of 4.0.
Fig. 6 Redundancy analysis (RDA) for the relationship between bacterial community and environmental variables (a), and between fungal community and environmental variables (b). SOC, soil organic content; TN, total nitrogen; TP, total phosphorus; AP, available phosphorus; SS, soil salinity; SWC, soil water content, BD, bulk density. The abbreviations are the same as in Figure 7.
Soil property Bacteria Fungi
RDA1 RDA2 R2 P RDA1 RDA2 R2 P
SOC 0.1519 0.9884 0.128 0.203 -0.924 0.3824 0.000 0.998
TN 0.3703 0.9289 0.112 0.273 0.3729 0.9279 0.147 0.203
TP 0.7140 0.7002 0.015 0.780 -0.6587 0.7524 0.200 0.093
NH4+-N 0.9286 -0.3710 0.220 0.067 0.4054 0.9141 0.053 0.563
NO3--N 0.4953 0.8687 0.107 0.310 0.7562 -0.6544 0.038 0.649
AP 0.9982 -0.0603 0.282 0.028* 0.7652 0.6437 0.302 0.020*
pH -0.9334 0.3588 0.654 0.010* -0.6964 -0.7176 0.017 0.804
SS 0.8473 -0.5310 0.430 0.052 -0.2629 0.9648 0.005 0.949
SWC -0.2024 -0.9793 0.213 0.081 0.9142 -0.4052 0.143 0.167
BD 0.8365 0.5480 0.089 0.305 0.9804 -0.1968 0.149 0.175
Table 3 Relationships between soil properties and two axes of redundancy analysis (RDA) for bacterial and fungal communities
Fig. 7 Correlations between bacterial community and soil properties (a) and between fungal community and soil properties (b). *, P<0.050 level; **, P<0.010 level; ***, P<0.001 level.
[1]   Bryant D A, Frigaard N U. 2006. Prokaryotic photosynthesis and phototrophy illuminated. Trends in Microbiology, 14(11): 488-496.
pmid: 16997562
[2]   Cao J J, Wang H R, Holden N M, et al. 2021. Soil properties and microbiome of annual and perennial cultivated grasslands on the Qinghai-Tibetan Plateau. Land Degradation & Development, 32(18): 5306-5321.
doi: 10.1002/ldr.v32.18
[3]   Cheng H, Wu B, Wei M, et al. 2021. Changes in community structure and metabolic function of soil bacteria depending on the type restoration processing in the degraded alpine grassland ecosystems in northern Tibet. Science of the Total Environment, 755: 142619, doi: 10.1016/j.scitotenv.2020.142619.
[4]   Clemmensen K E, Finlay R D, Dahlberg A, et al. 2015. Carbon sequestration is related to mycorrhizal fungal community shifts during long-term succession in boreal forests. New Phytologist, 205(4): 1525-1536.
doi: 10.1111/nph.13208 pmid: 25494880
[5]   de Carvalho T S, Jesus E D, Barlow J, et al. 2016. Land use intensification in the humid tropics increased both alpha and beta diversity of soil bacteria. Ecology, 97(10): 2760-2771.
doi: 10.1002/ecy.1513 pmid: 27859123
[6]   Deng J J, Yin Y, Zhu W X, et al. 2018. Variations in soil bacterial community diversity and structures among different revegetation types in the Baishilazi Nature Reserve. Frontiers in Microbiology, 9: 2874, doi: 10.3389/fmicb.2018.02874.
pmid: 30538689
[7]   Deng L, Liu G B, Shangguan Z P. 2014. Land-use conversion and changing soil carbon stocks in China's 'Grain-for-Green' program: A synthesis. Global Change Biology, 20(11): 3544-3556.
doi: 10.1111/gcb.12508 pmid: 24357470
[8]   Dou Y X, Liao J J, An S S. 2023. Importance of soil labile organic carbon fractions in shaping microbial community after vegetation restoration. Catena, 220: 106707, doi: 10.1016/j.catena.2022.106707.
[9]   Edgar R C. 2013. Uparse: Highly accurate Otu sequences from microbial amplicon reads. Nature Methods, 10: 996-998.
doi: 10.1038/nmeth.2604 pmid: 23955772
[10]   Feng X, Li J, Cheng W, et al. 2017. Evaluation of AMSR-E retrieval by detecting soil moisture decrease following massive dryland revegetation in the Loess Plateau, China. Remote Sensing of Environment, 196: 253-264.
doi: 10.1016/j.rse.2017.05.012
[11]   Feng X M, Fu B J, Piao S, et al. 2016. Revegetation in China's Loess Plateau is approaching sustainable water resource limits. Nature Climate Change, 6: 1019-1022.
doi: 10.1038/nclimate3092
[12]   Fierer N, Bradford M A, Jackson R B. 2007. Toward an ecological classification of soil bacteria. Ecology, 88(6): 1354-1364.
doi: 10.1890/05-1839 pmid: 17601128
[13]   Fu B J, Wang S, Liu Y, et al. 2017. Hydrogeomorphic ecosystem responses to natural and anthropogenic changes in the Loess Plateau of China. Annual Review of Earth and Planetary Sciences, 45: 223-243.
doi: 10.1146/earth.2017.45.issue-1
[14]   Heděnec P, Nilsson L O, Zheng H, et al. 2020. Mycorrhizal association of common European tree species shapes biomass and metabolic activity of bacterial and fungal communities in soil. Soil Biology and Biochemistry, 149: 107933, doi: 10.1016/j.soilbio.2020.107933.
[15]   Huang Q, Jiao F, Huang Y, et al. 2021. Response of soil fungal community composition and functions on the alteration of precipitation in the grassland of Loess Plateau. Science of the Total Environment, 751: 142273, doi: 10.1016/j.scitotenv.2020.142273.
[16]   Javed Z, Tripathi G D, Mishra M, et al. 2021. Actinomycetes-The microbial machinery for the organic-cycling, plant growth, and sustainable soil health. Biocatalysis and Agricultural Biotechnology, 31: 101893, doi: 10.1016/j.bcab.2020.101893.
[17]   Johnson S S, Hebsgaard M B, Christensen T R, et al. 2007. Ancient bacteria show evidence of DNA repair. Proceedings of the National Academy of Sciences, 104(36): 14401-14405.
[18]   Klatt C G, Liu Z F, Ludwig M, et al. 2013. Temporal metatranscriptomic patterning in phototrophic chloroflexi inhabiting a microbial mat in a geothermal spring. ISME Journal, 7: 1775-1789.
doi: 10.1038/ismej.2013.52 pmid: 23575369
[19]   Lauber C L, Strickland M S, Bradford M A, et al. 2008. The influence of soil properties on the structure of bacterial and fungal communities across land-use types. Soil Biology and Biochemistry, 40(9): 2407-2415.
doi: 10.1016/j.soilbio.2008.05.021
[20]   Lennon J T, Jones S E. 2011. Microbial seed banks: The ecological and evolutionary implications of dormancy. Nature Reviews Microbiology, 9: 119-130.
doi: 10.1038/nrmicro2504 pmid: 21233850
[21]   Li Y, Ma L, Wang J, et al. 2021. Soil faunal community composition alters nitrogen distribution in different land use types in the Loess Plateau, China. Applied Soil Ecology, 163: 103910, doi: 10.1016/j.apsoil.2021.103910.
[22]   Liu G F, Bai Z J, Cui G W, et al. 2022. Effects of land use on the soil microbial community in the Songnen Grassland of Northeast China. Frontiers in Microbiology, 13: 865184, doi: 10.3389/fmicb.2022.865184.
[23]   Liu R Q, Zhou X H, Wang J W, et al. 2019. Differential magnitude of rhizosphere effects on soil aggregation at three stages of subtropical secondary forest successions. Plant and Soil, 436: 365-380.
doi: 10.1007/s11104-019-03935-z
[24]   Mishra A, Singh D, Hathi Z, et al. 2023. Soil microbiome dynamics associated with conversion of tropical forests to different rubber based land use management systems. Applied Soil Ecology, 188: 104933, doi: 10.1016/j.apsoil.2023.104933.
[25]   Mukhopadhya I, Hansen R, El-Omar E M, et al. 2012. IBD-what role do Proteobacteria play? Nature Reviews Gastroenterology & Hepatology, 9: 219-230.
[26]   Nelson C E, Carlson C A. 2012. Tracking differential incorporation of dissolved organic carbon types among diverse lineages of Sargasso Sea bacterioplankton. Environmental Microbiology, 14: 1500-1516.
doi: 10.1111/j.1462-2920.2012.02738.x pmid: 22507662
[27]   Pii Y, Borruso L, Brusetti L, et al. 2016. The interaction between iron nutrition, plant species and soil type shapes the rhizosphere microbiome. Plant Physiology and Biochemistry, 99: 39-48.
doi: 10.1016/j.plaphy.2015.12.002 pmid: 26713550
[28]   Qin Y, Zhang X F, Adamowski J F, et al. 2021. Grassland grazing management altered soil properties and microbial β-diversity but not α-diversity on the Qinghai-Tibetan Plateau. Applied Soil Ecology, 167: 104032, doi: 10.1016/j.apsoil.2021.104032.
[29]   Ren C J, Zhou Z H, Guo Y X, et al. 2021. Contrasting patterns of microbial community and enzyme activity between rhizosphere and bulk soil along an elevation gradient. Catena, 196: 104921, doi: 10.1016/j.catena.2020.104921.
[30]   Rodrigues J L M, Pellizari V H, Mueller R, et al. 2013. Conversion of the Amazon rainforest to agriculture results in biotic homogenization of soil bacterial communities. Proceedings of the National Academy of Sciences of the United States of America, 110: 988-993.
[31]   Roper M, Seminara A, Bandi M M, et al. 2010. Dispersal of fungal spores on a cooperatively generated wind. Proceedings of the National Academy of Sciences of the United States of America, 107: 17474-17479.
[32]   Rutherford P M, McGill W B, Arocena J M, et al. 2007. Total nitrogen. In: Carter M R, Gregorich E G. Soil Sampling and Methods of Analysis (2nd ed.). Bosa Raton: CRC Press, 239-250.
[33]   Sawada K, Inagaki Y, Sugihara S, et al. 2021. Impacts of conversion from natural forest to cedar plantation on the structure and diversity of root-associated and soil microbial communities. Applied Soil Ecology, 167: 104027, doi: 10.1016/j.apsoil.2021.104027.
[34]   Sterkenburg E, Bahr A, Durling M B, et al. 2015. Changes in fungal communities along a boreal forest soil fertility gradient. New Phytologist, 207(4): 1145-1158.
doi: 10.1111/nph.13426 pmid: 25952659
[35]   Sun Y, Luo C, Jiang L, et al. 2020. Land-use changes alter soil bacterial composition and diversity in tropical forest soil in China. Science of the Total Environment, 712: 136526, doi: 10.1016/j.scitotenv.2020.136526.
[36]   Tedersoo L, Bahram M, Põlme S, et al. 2014. Global diversity and geography of soil fungi. Science, 346(6213): 1256688, doi: 10.1126/science.1256688.
[37]   Tian Q, Taniguchi T, Shi W Y, et al. 2017. Land-use types and soil chemical properties influence soil microbial communities in the semi-arid Loess Plateau region in China. Scientific Reports, 7: 45289, doi: 10.1038/srep45289.
pmid: 28349918
[38]   Treseder K K, Maltz M R, Hawkins B A, et al. 2014. Evolutionary histories of soil fungi are reflected in their large-scale biogeography. Ecology Letters, 17(9): 1086-1093.
doi: 10.1111/ele.12311 pmid: 24912000
[39]   Uroz S, Buee M, Deveau A, et al. 2016. Ecology of the forest microbiome: Highlights of temperate and boreal ecosystems. Soil Biology & Biochemistry, 103: 471-488.
doi: 10.1016/j.soilbio.2016.09.006
[40]   Wang K B, Zhang Y W, Tang Z S, et al. 2019. Effects of grassland afforestation on structure and function of soil bacterial and fungal communities. Science of the Total Environment, 676: 396-406.
doi: 10.1016/j.scitotenv.2019.04.259
[41]   Widdig M, Schleuss P M, et al. 2020. Effects of nitrogen and phosphorus addition on microbial community composition and element cycling in a grassland soil. Soil Biology & Biochemistry, 151: 108041, doi: 10.1016/j.soilbio.2020.108041.
[42]   Wu H F, Hu B A, Cheng X Q, et al. 2023. Relative importance of influencing factor-driven soil enzyme activity during the early plantation stage in the hilly-gully loess regions. Land Degradation & Development, 34: 2483-2493.
doi: 10.1002/ldr.v34.9
[43]   Wubet T, Christ S, Schöning I, et al. 2012. Differences in soil fungal communities between European beech (Fagus Sylvatica L.) dominated forests are related to soil and understory vegetation. PLoS ONE, 7(10): e47500, doi: 10.1371/journal.pone.0047500.
[44]   Xiao B, Veste M. 2017. Moss-dominated biocrusts increase soil microbial abundance and community diversity and improve soil fertility in semi-arid climates on the Loess Plateau of China. Applied Soil Ecology, 117-118: 165-177.
[45]   Xu A, Liu J, Guo Z, et al. 2021. Soil microbial community composition but not diversity is affected by land-use types in the agro-pastoral ecotone undergoing frequent conversions between cropland and grassland. Geoderma, 401: 115165, doi: 10.1016/j.geoderma.2021.115165.
[46]   Xu A, Guo Z, Pan K, et al. 2023. Increasing land-use durations enhance soil microbial deterministic processes and network complexity and stability in an ecotone. Applied Soil Ecology, 181: 104630, doi: 10.1016/j.apsoil.2022.104630.
[47]   Xu J, Liu S J, Song S R, et al. 2018. Arbuscular mycorrhizal fungi influence decomposition and the associated soil microbial community under different soil phosphorus availability. Soil Biology & Biochemistry, 120: 181-190.
doi: 10.1016/j.soilbio.2018.02.010
[48]   Xu M P, Lu X Q, Xu Y D, et al. 2020. Dynamics of bacterial community in litter and soil along a chronosequence of Robinia pseudoacacia plantations. Science of the Total Environment, 703: 135613, doi: 10.1016/j.scitotenv.2019.135613.
[49]   Xu Y, Zhong Z, Zhang W, et al. 2019. Responses of soil nosz-type denitrifying microbial communities to the various land-use types of the Loess Plateau, China. Soil and Tillage Research, 195: 104378, doi: 10.1016/j.still.2019.104378.
[50]   Yang C, Wang X, Miao F, et al. 2020a. Assessing the effect of soil salinization on soil microbial respiration and diversities under incubation conditions. Applied Soil Ecology, 155: 103671, doi: 10.1016/j.apsoil.2020.103671.
[51]   Yang F, Niu K C, Collins C G, et al. 2019. Grazing practices affect the soil microbial community composition in a Tibetan alpine meadow. Land Degradation & Development, 30: 49-59.
doi: 10.1002/ldr.v30.1
[52]   Yang X, You L C, Hu H W, et al. 2022. Conversion of grassland to cropland altered soil nitrogen-related microbial communities at large scales. Science of the Total Environment, 816: 151645, doi: 10.1016/j.scitotenv.2021.151645.
[53]   Yang Y, Cheng H, Liu L, et al. 2020b. Comparison of soil microbial community between planted woodland and natural grass vegetation on the Loess Plateau. Forest Ecology and Management, 460: 117817, doi: 10.1016/j.foreco.2019.117817.
[54]   Yang Y, Chai Y B, Xie H J, et al. 2023. Responses of soil microbial diversity, network complexity and multifunctionality to three land-use changes. Science of the Total Environment, 859: 160255, doi: 10.1016/j.scitotenv.2022.160255.
[55]   Yu Z, Liang K N, Huang G H, et al. 2021. Soil bacterial community shifts are driven by soil nutrient availability along a teak plantation chronosequence in tropical forests in China. Biology, 10(12): 1329, doi: 10.3390/biology10121329.
[56]   Zeng Q C, Liu Y, Xiao L, et al. 2020. Climate and soil properties regulate soil fungal communities on the Loess Plateau. Pedobiologia, 81-82(1): 150668, doi: 10.1016/j.pedobi.2020.150668.
[57]   Zhalnina K, Dias R, et al. 2015. Soil pH determines microbial diversity and composition in the park grass experiment. Microbial Ecology, 69: 395-406.
doi: 10.1007/s00248-014-0530-2 pmid: 25395291
[58]   Zhang L, Lv J. 2021. The impact of land-use change on the soil bacterial community in the Loess Plateau, China. Journal of Arid Environments, 188: 104469, doi: 10.1016/j.jaridenv.2021.104469.
[59]   Zhang P, Guan P T, Hao C, et al. 2022. Changes in assembly processes of soil microbial communities in forest-to-cropland conversion in Changbai Mountains, northeastern China. Science of the Total Environment, 818: 151738, doi: 10.1016/j.scitotenv.2021.151738.
[60]   Zhang X F, Feng Q, Adamowski J F, et al. 2023. Conversion of grassland to abandoned land and afforested land alters soil bacterial and fungal communities on the Loess Plateau. Applied Soil Ecology, 183: 104758, doi:10.1016/j.apsoil.2022.104758.
[61]   Zhou H, Zhang D, Jiang Z, et al. 2019. Changes in the soil microbial communities of alpine steppe at Qinghai-Tibetan Plateau under different degradation levels. Science of the Total Environment, 651: 2281-2291.
doi: 10.1016/j.scitotenv.2018.09.336
[62]   Zhou Z H, Wang C K, Luo Y Q. 2020. Meta-analysis of the impacts of global change factors on soil microbial diversity and functionality. Nature Communications, 11: 3072, doi: 10.1038/s41467-020-16881-7.
pmid: 32555185
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