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Journal of Arid Land
Journal of Arid Land  2018, Vol. 10 Issue (1): 152-167    DOI: 10.1007/s40333-017-0033-3     CSTR: 32276.14.s40333-017-0033-3
Orginal Article     
Desiccation tolerance in bryophytes: the rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism
Bei GAO1,2, Daoyuan ZHANG1,*(), Xiaoshuang LI1, Honglan YANG1, Yuqing LIANG1, Moxian CHEN2, Yuanming ZHANG1, Jianhua ZHANG2,3, WOOD Andrew4
1 Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China
2 School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China
3 Department of Biology, Faculty of Science, Hong Kong Baptist University, Hong Kong, China
4 Department of Plant Biology, Southern Illinois University-Carbondale, Carbondale, IL 62901-6509, USA
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Abstract  

Bryum argenteum Hedw. is a desiccation tolerant bryophyte and belongs to one of the most important components of the biological soil crusts (BSCs) found in the deserts of Central Asia. Limited information is available on rehydration-responsive proteins in desiccation tolerant plants. As a complement to our previous research analyzing the rehydration transcriptome, we present a parallel quantitative proteomic effort to study rehydration-responsive proteins. Bryophyte gametophores were desiccated (Dry) and rehydrated for 2 h (R2) and 24 h (R24). Proteins from Dry, R2 and R24 gametophores were labeled by isobaric tags for relative and absolute quantitation (iTRAQ) to determine the relative abundance of rehydration-responsive proteins. A total of 5503 non-redundant protein sequences were identified and 4772 (86.7%) protein sequences were annotated using Gene Ontology (GO) terms and Pfam classifications. Upon rehydration 239 proteins were elevated and 461 proteins were reduced as compared to the desiccated protein sample. Differentially up-regulated proteins were classified into a number of categories including reactive oxygen species scavenging enzymes, detoxifying enzymes, Late Embryogenesis Abundant (LEA) proteins, heat shock proteins, proteasome components and proteases, and photosynthesis and translation related proteins. Furthermore, the results of the correlation between transcriptome and proteome revealed the discordant changes in the expression between protein and mRNA.

Key words： desiccation tolerance      Bryum      Physcomitrella patens      proteome      iTRAQ      rehydration     
Received: 15 August 2016      Published: 10 February 2018
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Bei GAO
Daoyuan ZHANG
Xiaoshuang LI
Honglan YANG
Yuqing LIANG
Moxian CHEN
Yuanming ZHANG
Jianhua ZHANG
WOOD Andrew
Cite this article:

Bei GAO, Daoyuan ZHANG, Xiaoshuang LI, Honglan YANG, Yuqing LIANG, Moxian CHEN, Yuanming ZHANG, Jianhua ZHANG, WOOD Andrew. Desiccation tolerance in bryophytes: the rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism. Journal of Arid Land, 2018, 10(1): 152-167.

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http://jal.xjegi.com/10.1007/s40333-017-0033-3     OR     http://jal.xjegi.com/Y2018/V10/I1/152

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