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Journal of Arid Land  2019, Vol. 11 Issue (2): 292-305    DOI: 10.1007/s40333-019-0007-8
Orginal Article     
Exogenously applied glycinebetaine induced alteration in some key physio-biochemical attributes and plant anatomical features in water stressed oat (Avena sativa L.) plants
SHEHZADI Anum1, A AKRAM Nudrat1,*(), ALI Ayaz1, ASHRAF Muhammad2,3
1 Department of Botany, Government College University, Faisalabad 38040, Pakistan
2 Pakistan Science Foundation, Islamabad 44000, Pakistan
3 Department of Botany, King Saud University, Riyadh 11564, Saudi Arabia
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Although exogenous application of glycinebetaine (GB) is widely reported to regulate a myriad of physio-biochemical attributes in plants under stressful environments including drought stress, there is little information available in the literature on how and up to what extent GB can induce changes in anatomical features in water starved plants. Thus, the present research work was conducted to assess the GB-induced changes in growth, physio-biochemical, and anatomical characteristics in two cultivars (CK-1 and F-411) of oat (Avena sativa L.) under limited water supply. After exposure to water stress, a considerable reduction was observed in plant growth in terms of lengths and weights of shoot and roots, leaf mesophyll thickness, leaf midrib thickness, root cortex thickness, root diameter, stem diameter, stem phloem area, and stem vascular bundle area in both oat cultivars. However, water stress resulted in a significant increase in leaf total phenolics, hydrogen peroxide (H2O2), ascorbic acid (AsA), GB contents, activities of enzymes (CAT, SOD and POD), total soluble proteins, leaf epidermis (abaxial and adaxial) thickness, bulliform cell area, sclerenchyma thickness, root endodermis and epidermis thickness, root metaxylem area, stem metaxylem area and stem sclerenchyma thickness in both oat cultivars. Foliar-applied 100 mM GB suppressed H2O2 contents, while improved growth attributes, free proline and GB contents, activity of SOD enzyme, leaf abaxial epidermis thickness, leaf bulliform cell area, leaf midrib thickness, leaf sclerenchyma thickness, root cortex thickness, root endodermis, epidermis thickness, root stele diameter, stem diameter, stem epidermis thickness, stem metaxylem area, and stem phloem and vascular bundle area in both oat cultivars. For both oat cultivars, CK-1 was superior to F-411 in leaf abaxial epidermis thickness, leaf mesophyll, leaf sclerenchyma, root metaxylem area, stem diameter, stem epidermis, sclerenchyma thickness, stem metaxylem area, and stem vascular bundle area. Overall, both oat cultivars showed inconsistent behavior to water stress and foliar-applied GB in terms of different physio-biochemical attributes, however, CK-1 was superior to F-411 in a number of anatomical features of leaf, root, and stem.

Key wordsdrought tolerance      anatomical features      oat      glycinebetaine      antioxidants     
Received: 14 October 2017      Published: 10 April 2019
Corresponding Authors: A AKRAM Nudrat     E-mail:
Cite this article:

SHEHZADI Anum, A AKRAM Nudrat, ALI Ayaz, ASHRAF Muhammad. Exogenously applied glycinebetaine induced alteration in some key physio-biochemical attributes and plant anatomical features in water stressed oat (Avena sativa L.) plants. Journal of Arid Land, 2019, 11(2): 292-305.

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[1] Abdel C G, Al-Rawi I M T.2011. Anatomical alteration in response to irrigation and water stress in some legume crops. American Journal of Experimental Agriculture, 1(4): 231-264.
[2] Ahmad P, Rasool S, Gul A, et al.2016. Jasmonates: Multifunctional roles in stress tolerance. Frontiers in Plant Science, 7: 813.
[3] Ahmed M F E M, Mac D M, Bashir A A G.2011. Effect of water stress at different periods on seed yield and water use efficiency of guar under Shambat conditions. Agricultural Sciences, 2(3): 262-266.
[4] Akram N A, Shahbaz M, Ashraf M.2007. Relationship of photosynthetic capacity and proline accumulation with the growth of differently adapted populations of two potential grasses (Cynodon dactylon (L.) Pers. and Cenchrus ciliaris L.) to drought stress. Pakistan Journal of Botany, 39(3): 777-786.
[5] Akram N A, Noreen S, Noreen T, et al.2015. Exogenous application of trehalose alters growth, physiology and nutrient composition in radish (Raphanus sativus L.) plants under water deficit conditions. Brazilian Journal of Botany, 38(3): 431-439.
[6] Akram N A, Shafiq S, Ashraf M, et al.2016. Drought-induced anatomical changes in radish (Raphanus sativus L.) leaves supplied with trehalose through different modes. Arid Land Research and Management, 30(4): 412-420.
[7] Aldesuquy H S, Abbas M A, Abo-Hamed S A, et al.2013. Does glycine betaine and salicylic acid ameliorate the negative effect of drought on wheat by regulating osmotic adjustment through solutes accumulation? Journal of Stress Physiology and Biochemistry, 9(3): 5-22.
[8] Arnon D I.1949. Copper enzymes in isolated chloroplast, polyphenoloxidase in Beta vulgaris L. Journal of Plant Physiology, 24(1): 1-15.
[9] Ashikari M, Feng-Ma J.2015. Exploring the power of plants to overcome environmental stresses. Rice (NY), 8: 10.
[10] Ashraf M, Foolad M R.2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59(2): 206-216.
[11] Ashraf M, Athar H R, Harris P J C, et al.2008. Some prospective strategies for improving crop salt tolerance. Advances in Agronomy, 97: 45-110.
[12] Ashraf M.2010. Inducing drought tolerance in plants: recent advances. Biotechnology Advances, 28(1): 169-183.
[13] Ashraf M, Akram N A, Al-Qurainy F, et al.2011. Drought tolerance: Roles of organic osmolytes, growth regulators, and mineral nutrients. Advances in Agronomy, 111: 249-296.
[14] Ashraf M, Harris P J C.2013. Photosynthesis under stressful environments: An overview. Photosynthetica, 51(2): 163-190.
[15] Balsamo R A, Willigen C V, Baue A M, et al.2006. Drought tolerance of selected Eragrostis species correlates with leaf tensile properties. Annals of Botany, 97(6): 985-991.
[16] Bates L S, Waldren R P, Teare I D.1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39(1): 205-207.
[17] Behnamnia M, Kalantari K M, Rezanejad F.2009. Exogenous application of brassinosteroid alleviates drought-induced oxidative stress in Lycopersicon esculentum L. General and Applied Plant Physiology, 35(1-2): 22-34.
[18] Bloch D, Hoffmann C M, Märander B.2006. Impact of water supply on photosynthesis, water use and carbon isotope discrimination of sugar beet genotypes. European Journal of Agronomy, 24(3): 218-225.
[19] Boughalleb F, Abdellaoui R, Brahim N, et al.2014. Anatomical adaptations of Astragalus gombiformis Pomel. under drought stress. Central European Journal of Biology, 9(12): 1215-1225.
[20] Bradford M M.1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annual Biochemistry, 72(1-2): 248-254.
[21] Carmak I, Horst W J.1991. Effects of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum, 83(3): 463-468.
[22] Chance B, Maehly A C.1955. Assay of catalases and peroxidases. Methods in Enzymology, 2: 764-775.
[23] Cha-um S, Kirdmanee C.2010. Effect of glycinebetaine on proline, water use, and photosynthetic efficiencies, and growth of rice seedlings under salt stress. Turkish Journal of Agriculture and Forestry, 34: 517-527.
[24] Chaves M M, Oliveira M M.2004. Mechanisms underlying plant resilience to water deficits: Prospects for water-saving agriculture. Journal of Experimental Botany, 55(407): 2365-2384.
[25] Chen T H H, Murata N.2008. Glycinebetaine: an effective protectant against abiotic stress in plants. Trends in Plant Science, 13(9): 499-505.
[26] Farooq M, Basra S M A, Wahid A, et al.2008. Physiological role of exogenously applied glycinebetaine to improve drought tolerance in fine grain aromatic rice (Oryza sativa L.). Journal of Agronomy and Crop Sciences, 194(5): 325-333.
[27] Ghorbanli M, Gafarabad M, Amirkian T, et al.2013. Investigation of proline, total protein, chlorophyll, ascorbate and dehydroascorbate changes under drought stress in Akria and Mobil tomato cultivars. Iranian Journal of Plant Physiology, 3(2): 651-658.
[28] Giannopolitis C N, Ries S K.1977. Superoxide dismutase. I. Occurrence in higher plants. Plant Physiology, 59(2): 309-314.
[29] Giri J.2011. Glycinebetaine and biotic stress tolerance in plants. Plant Signaling & Behavior, 6(11): 1746-1751.
[30] Grieve C M, Grattan S R.1983. Rapid assay for determination of water soluble quaternary ammonium compounds. Plant and Soil, 70(2): 303-307.
[31] Hameed A, Goher M, Iqbal N.2013. Drought induced programmed cell death and associated changes in antioxidants, proteases, and lipid peroxidation in wheat leaves. Biologia Plantarum, 57(2): 370-374.
[32] Hamidou F, Zombre G, Diouf O, et al.2007. Physiological, biochemical and agro-morphological responses of five cowpea genotypes (Vigna unguiculata (L.) Walp.) to water deficit under glasshouse conditions. Biotechnology, Agronomie, Societie and Environment, 11(3): 225-234.
[33] Hasheminasab H, Assad M T, Aliakbari A, et al.2012. Influence of drought stress on oxidative damage and antioxidant defense systems in tolerant and susceptible wheat genotypes. Journal of Agricultural Science, 4(8): 20-30.
[34] Hossain M A, Fujita M.2010. Evidence for a role of exogenous glycinebetaine and proline in antioxidant defense and methylglyoxal detoxification systems in mung bean seedlings under salt stress. Physiology and Molecular Biology of Plants, 16(1): 19-29.
[35] Hussain M, Malik M A, Farooq M, et al.2008. Improving drought tolerance by exogenous application of glycinebetaine and salicylic acid in sunflower. Journal of Agronomy and Crop Science 194(3): 193-199.
[36] Iqbal N, Ashraf M, Ashraf M Y.2009. Influence of exogenous glycine betaine on gas exchange and biomass production in sunflower (Helianthus annuus L.) under water limited conditions. Journal of Agronomy and Crop Science, 195(6): 420-426.
[37] Jatoi W A, Baloch M J, Kumbhar M B, et al.2011. Effect of water stress on physiological and yield parameters at anthesis stage in elite spring wheat cultivars. Sarhad Journal of Agriculture, 27: 59-65.
[38] Julkunen-Tiitto R.1985. Phenolic constituents in the leaves of north willows: methods for the analysis of certain phenolics. Journal of Agricultural and Food Chemistry, 33(2): 213-217.
[39] Khan M A, Shirazi M U, Khan M A, et al.2009. Role of proline, K/Na ratio and chlorophyll content in salt tolerance of wheat (Triticum aestivum L.). Pakistan Journal of Botany, 41(2): 633-638.
[40] Kumar R R, Karajol K, Naik G R.2011. Effect of polyethylene glycol induced water stress on physiological and biochemical responses in pigeon pea (Cajanus cajan L. Mill sp.). Recent Research in Science & Technology, 3: 148-152.
[41] Lum M S, Hanafi M M, Rafii Y M, et al.2014. Effect of drought stress on growth, proline and antioxidant enzyme activities of upland rice. Journal of Animal and Plant Sciences, 24(5): 1487-1493.
[42] Ma Q Q, Wang W, Li Y H, et al.2006. Alleviation of photoinhibition in drought-stressed wheat (Triticum aestivum L.) by foliar-applied glycinebetaine. Journal of Plant Physiology, 163(2): 165-175.
[43] Ma X L, Wang Y J, Xie S L, et al.2007. Glycinebetaine application ameliorates negative effects of drought stress in tobacco. Russian Journal of Plant Physiology, 54: 472-479.
[44] Mafakheri A, Siosemardeh A, Bahramnejad B, et al.2010. Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Australian Journal of Crop Science, 4(8): 580-585.
[45] Mahmood T, Ashraf M, Shahbaz M.2009. Does exogenous application of glycinebetaine as a pre-sowing seed treatment improve growth and regulate some key physiological attributes in wheat plants grown under water deficit conditions? Pakistan Journal of Botany, 41(3): 1291-1302.
[46] Makbul S, Coskuncelebi K, Turkmen Z, et al.2006. Morphology and anatomy of Scrophularia L. (Scrophulariaceae) taxa from NE anatolia. Acta Biologica Cracoviensia Series Botanica, 48(1): 33-43.
[47] Makbul S, Türkmen Z, Coskuncelebi K, et al.2008. Anatomical and pollen characters in the genus Epilobium L. (Onagraceae) from northeast anatolia. Acta Biologica Cracoviensia Series Botanica, 50(1): 51-62.
[48] Makbul S, Guler N S, Durmus N, et al.2011. Changes in anatomical and physiological parameters of soybean under drought stress. Turkish Journal of Botany, 35: 369-377.
[49] Mukherjee S P, Chouduri M A.1983. Implications of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiologia Plantarum, 58(2): 166-170.
[50] Mwenye O J, van Rensburg L, van Biljon A, et al.2016. The role of proline and root traits on selection for drought-stress tolerance in soybeans: A review. South African Journal of Plant and Soil, 33(4): 245-256.
[51] Nawaz K, Ashraf M.2010. Exogenous application of glycinebetaine modulates activities of antioxidants in maize plants subjected to salt stress. Journal of Agronomy and Crop Science, 196(1): 28-37.
[52] Nawaz T, Hameed M, Ashraf M, et al.2013. Modifications in root and stem anatomy for water conservation in some diverse blue panic (Panicum antidotale Retz.) ecotypes under drought stress. Arid Land Research and Management, 27(3): 286-297.
[53] Nemeskéri E, Sárdi é, Remenyik J, et al.2010. Study of the defensive mechanism against drought in French bean (Phaseolus vulgaris L.) varieties. Acta Physiologia Plantarum, 32(6): 1125-1134.
[54] Omar A A.2012. Impact of drought stress on germination and seedling growth parameters of some wheat cultivars. Life Science Journal, 9(1): 590-598.
[55] Ozorgucu B, Gemici Y, Turkan I.1991. Comparative Plant Anatomy. Ïzmir: Ege University Faculty of Science Publication No: 129. (in Turkish)
[56] Pratap V, Sharma Y K.2010. Impact of osmotic stress on seed germination and seedling growth in black gram (Phaseolus mungo). Journal of Environmental Biology, 31(5): 721-726.
[57] Raza M A S, Saleem M F, Khan I H, et al.2012. Evaluating the drought stress tolerance efficiency of wheat (Triticum aestivum L.) cultivars. Russian Journal of Agricultural and Socio-Economic Science, 12(12): 41-46.
[58] Rezaei M A, Kaviani B, Masouleh A K.2012. The effect of exogenous glycine betaine on yield of soybean (Glycine max (L.) Merr.) in two contrasting cultivars Pershing and DPX under soil salinity stress. Plant Omics Journal, 5(2): 87-93.
[59] Ruzin S E.1999. Plant Microtechnique and Microscopy. New York: Oxford University Press, 322.
[60] Sadiq M, Akram N A, Ashraf M.2017. Foliar applications of alpha-tocopherol improve the composition of fresh pods of Vigna radiata subjected to water deficiency. Turkish Journal of Botany, 41: 244-252.
[61] Saeed N, Maqbool N, Haseeb M, et al.2016. Morpho-anatomical changes in roots of chickpea (Cicer arietinum L.) under drought stress condition. Journal of Agricultural Science and Technology, 6: 1-9.
[62] Seki M, Kamei A, Yamaguchi-Shinozaki K, et al.2003. Molecular responses to drought, salinity and frost: common and different paths for plant protection. Current Opinion in Biotechnology, 14(2): 194-199.
[63] Shafiq S, Akram N A, Ashraf M.2015. Does exogenously-applied trehalose alter oxidative defense system in the edible part of radish (Raphanus sativus L.) under water-deficit conditions? Scientia Horticulturae, 185: 68-75.
[64] Shahbaz M, Masood Y, Ashraf M.2011. Is foliar-applied glycinebetaine effective in mitigating the adverse effects of drought stress on wheat (Triticum aestivum L.)? Journal of Applied Botany and Food Quality, 84(2): 192-199.
[65] Snedecor G W, Cochran W G.1980. Statistical Methods (7th ed.). Ames: Iowa State University Press, 97.
[66] Subbarao G V, Wheeler R M, Levine L H, et al.2001. Glycine betaine accumulation, ionic and water relations of red-beet at contrasting levels of sodium supply. Journal of Plant Physiology, 158(6): 767-776.
[67] Vasellati V, Oesterheld M, Medan D, et al.2001. Effects of flooding and drought on the anatomy of Paspalum dilatatum. Annals of Botany, 88(3): 355-360.
[68] Velikova V, Yordanov I, Edreva A.2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Science, 151(1): 59-66.
[69] Wang W, Vinocur B, Altman A.2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta, 218(1): 1-14.
[70] Zhang L, Gao M, Hu J, et al.2012. Modulation role of abscisic acid (ABA) on growth, water relations and glycinebetaine metabolism in two maize (Zea mays L.) cultivars under drought stress. International Journal of Molecular Sciences, 13: 3189-3202.
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