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Journal of Arid Land  2017, Vol. 9 Issue (1): 153-159    DOI: 10.1007/s40333-016-0026-7
Orginal Article     
Fecal microbiota of three bactrian camels (Camelus ferus and Camelus bactrianus) in China by high throughput sequencing of the V3-V4 region of the 16S rRNA gene
Lei YUAN1, Aladaer QI2,3,*(), Yun CHENG1, Guli SAGEN1, Yuan QU4, Bin LIU3
1 Xinjiang Lop Nor Wild Camel National Nature Reserve, Urumqi 830011, China
2 Animal Science Department, Xinjiang Agricultural University, Urumqi 830052, China
3 Key Laboratory of Biogeography and Bioresources in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China
4 The People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China;
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This study aimed to reveal the microbial diversity in the fecal samples of bactrian camels using the 16S rRNA sequencing analysis on the Illumina MiSeq platform. Three fecal samples were collected from two geographical regions in China. Operational taxonomic unit (OTU) clustering was performed by identifying an OTU at 97% sequence identity. The alpha and beta diversities were applied to estimate the differences in microbial diversity among the three fecal samples. Totally, 4409, 3151 and 4075 OTUs in the fecal samples were identified in the Lop Nor wild camel (Camelus ferus), the domestic camel (C. bactrianus) and Dunhuang wild camel (C. ferus), respectively. The majority of bactreria were affiliated with phylum Firmicutes and Bacteroidetes in the three samples. The wild camels had higher gastrointestinal tract microbial diversity than the domestic one, while the microbial composition of the Lop Nor wild camel shared higher similarity with domestic camel at the genus and family levels than that of the Dunhuang wild camel did. Our results may provide a theoretical basis for assessing their health conditions and may thus be useful for protecting the critically endangered species of C. ferus.

Key wordsbactrian camels      Camelus ferus      fecal microbiota      16S rRNA      high-throughput sequencing     
Received: 25 March 2016      Published: 31 July 2017
Corresponding Authors: Aladaer QI     E-mail:
Cite this article:

Lei YUAN, Aladaer QI, Yun CHENG, Guli SAGEN, Yuan QU, Bin LIU. Fecal microbiota of three bactrian camels (Camelus ferus and Camelus bactrianus) in China by high throughput sequencing of the V3-V4 region of the 16S rRNA gene. Journal of Arid Land, 2017, 9(1): 153-159.

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