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Journal of Arid Land  2017, Vol. 9 Issue (2): 278-286    DOI: 10.1007/s40333-017-0052-0
Research Article     
Bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China
Wei ZHANG1,2, Gaosen ZHANG1,2, Xiukun WU1,2, Guangxiu LIU1,2,*(), Zhibao DONG1,2, Jianjun QU1,2,3, Yun WANG1,2, Tuo CHEN1,2
1 Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China
2 Gansu Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Lanzhou 730000, China
3 Dunhuang Gobi and Desert Ecology and Environment Research Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Dunhuang 736200, China
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Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China was analyzed using high-throughput amplicon pyrosequencing analysis. The sequences of the most abundant OUTs (Operational Taxonomic Units) in the sediment of Crescent Moon Spring were compared with the sequences of those most abundant OUTs of various origins from NCBI GenBank database to detect the origins of bacteria in the sediment of Crescent Moon Spring. Also, bacterial compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems (including desert lakes) worldwide were compared using cluster analysis to determine the possible factors affecting bacterial compositions. In total, 11,855 sequences were obtained and 30 phyla were identified. At the phylum level, the dominant phylum was Proteobacteria with α-Proteobacteria being the first dominant class and the second dominant phylum was Planctomycetes. Our finding that α-Proteobacteria being the first dominant class of Proteobacteria and Planctomycetes being the second dominant phyla are somewhat contradictory with reports from other desert lake sediments. This difference could be resulted from water hydration and conductivity, as well as oligotrophic conditions of Crescent Moon Spring. At the genus level, Rhodobacter, Caldilinea, Planctomyces, and Porphyrobacter were the dominant genera in the sediment of Crescent Moon Spring. Comparisons on sequences of the most abundant OUTs (including OTU3615, OTU6535, and OTU6646) between sediment of Crescent Moon Spring and various origins from NCBI GenBank database indicate that the origins of bacteria in the sediment of Crescent Moon Spring are likely from the underground water. Furthermore, cluster analysis on comparisons of bacteria compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems (including desert lakes) worldwide shows that at regional scales, bacterial compositions may be mainly affected by geographical patterns, precipitation amounts, and pH values. Collectively, our results provide new knowledge on the bacterial diversity in desert lake ecosystems.

Key wordsbacterial diversity      pyrosequencing analysis      desert lake      desert and lake ecosystems      Crescent Moon Spring     
Received: 23 December 2015      Published: 20 April 2017
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Wei ZHANG, Gaosen ZHANG, Xiukun WU, Guangxiu LIU, Zhibao DONG, Jianjun QU, Yun WANG, Tuo CHEN. Bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China. Journal of Arid Land, 2017, 9(2): 278-286.

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