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Journal of Arid Land  2017, Vol. 9 Issue (2): 278-286    DOI: 10.1007/s40333-017-0052-0
Research Article     
Bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China
Wei ZHANG1,2, Gaosen ZHANG1,2, Xiukun WU1,2, Guangxiu LIU1,2,*(), Zhibao DONG1,2, Jianjun QU1,2,3, Yun WANG1,2, Tuo CHEN1,2
1 Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China
2 Gansu Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Lanzhou 730000, China
3 Dunhuang Gobi and Desert Ecology and Environment Research Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Dunhuang 736200, China
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Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China was analyzed using high-throughput amplicon pyrosequencing analysis. The sequences of the most abundant OUTs (Operational Taxonomic Units) in the sediment of Crescent Moon Spring were compared with the sequences of those most abundant OUTs of various origins from NCBI GenBank database to detect the origins of bacteria in the sediment of Crescent Moon Spring. Also, bacterial compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems (including desert lakes) worldwide were compared using cluster analysis to determine the possible factors affecting bacterial compositions. In total, 11,855 sequences were obtained and 30 phyla were identified. At the phylum level, the dominant phylum was Proteobacteria with α-Proteobacteria being the first dominant class and the second dominant phylum was Planctomycetes. Our finding that α-Proteobacteria being the first dominant class of Proteobacteria and Planctomycetes being the second dominant phyla are somewhat contradictory with reports from other desert lake sediments. This difference could be resulted from water hydration and conductivity, as well as oligotrophic conditions of Crescent Moon Spring. At the genus level, Rhodobacter, Caldilinea, Planctomyces, and Porphyrobacter were the dominant genera in the sediment of Crescent Moon Spring. Comparisons on sequences of the most abundant OUTs (including OTU3615, OTU6535, and OTU6646) between sediment of Crescent Moon Spring and various origins from NCBI GenBank database indicate that the origins of bacteria in the sediment of Crescent Moon Spring are likely from the underground water. Furthermore, cluster analysis on comparisons of bacteria compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems (including desert lakes) worldwide shows that at regional scales, bacterial compositions may be mainly affected by geographical patterns, precipitation amounts, and pH values. Collectively, our results provide new knowledge on the bacterial diversity in desert lake ecosystems.

Key wordsbacterial diversity      pyrosequencing analysis      desert lake      desert and lake ecosystems      Crescent Moon Spring     
Received: 23 December 2015      Published: 20 April 2017
Corresponding Authors: Guangxiu LIU     E-mail:
Cite this article:

Wei ZHANG, Gaosen ZHANG, Xiukun WU, Guangxiu LIU, Zhibao DONG, Jianjun QU, Yun WANG, Tuo CHEN. Bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China. Journal of Arid Land, 2017, 9(2): 278-286.

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1 Abed R M M, Ramette A, Hübner V, et al.2012. Microbial diversity of eolian dust sources from saline lake sediments and biological soil crusts in arid Southern Australia. FEMS Microbiology Ecology, 80(2): 294-304.
2 Azua-Bustos A, Urrejola C, Vicu?a R.2012. Life at the dry edge: microorganisms of the Atacama Desert. FEBS Letters, 586(18): 2939-2945.
3 Bhatnagar A, Bhatnagar M.2005. Microbial diversity in desert ecosystems. Current Science, 89(1): 91-100.
4 Bhatt H H, Pasricha R, Upasani V N.2016. Isolation and characterization of a halophilic cyanobacterium Euhalothece SLVH01 from Sambhar salt lake, India. International Journal of Current Microbiology and Applied Sciences, 5(2): 215-224.
5 Cai H Z, Cheng Z J.1990. Some blue-green algae collected from the Crescent Moon Spring in Dunhuang. Journal of Northwest Normal University, (4): 60-63. (in Chinese)
6 Chanal A, Chapon V, Benzerara K, et al.2006. The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria. Environmental Microbiology, 8(3): 514-525.
7 Dong H L, Zhang G X, Jiang H C, et al.2006. Microbial diversity in sediments of saline Qinghai Lake, China: linking geochemical controls to microbial ecology. Microbial Ecology, 51(1): 65-82.
8 Dong J H.2007. Investigation on the degradation of natural heritage of Singing Sand Mountain and Crescent Moon Spring at Dunhuang city, China. Chinese Journal of Population Resources and Environment, 5(4): 85-91.
9 Dong Z B, Qian G G, Yan P, et al.2010. Gravel bodies in the Kumtagh Desert and their geomorphological implications. Environmental Earth Sciences, 59(8): 1771-1779.
10 Eldridge D J, Woodhouse J N, Curlevski N J A, et al.2015. Soil-foraging animals alter the composition and co-occurrence of microbial communities in a desert shrubland. The ISME Journal, 9(12): 2671-2681.
11 Fierer N, Strickland M S, Liptzin D, et al.2009. Global patterns in belowground communities. Ecology Letters, 12(11): 1238-1249.
12 Gans J, Wolinsky M, Dunbar J.2005. Computational improvements reveal great bacterial diversity and high metal toxicity in soil. Science, 309(5739): 1387-1390.
13 He K J, Qi Y, Huang Y M, et al.2016. Response of aboveground biomass and diversity to nitrogen addition-a five-year experiment in semi-arid grassland of Inner Mongolia, China. Scientific Reports, 6: 31919.
14 He X M, Lv G H, Qin L, et al.2015. Effects of simulated nitrogen deposition on soil respiration in a Populus euphratica community in the Ebinur Lake area, a desert ecosystem of northwestern China. PLoS ONE, 10(9): e0137827, doi: 10.1371/journal.pone.0137827.
15 Hedi A, Sadfi N, Fardeau M L, et al.2009. Studies on the biodiversity of halophilic microorganisms isolated from El-Djerid Salt Lake (Tunisia) under aerobic conditions. International Journal of Microbiology, 2009, 731786, doi: 10.1155/2009/731786.
16 Jiao J J.2010. Crescent Moon Spring: a disappearing natural wonder in the Gobi desert, China. Groundwater, 48(1): 159-163.
17 K?berl M, Müller H, Ramadan E M, et al.2011. Desert farming benefits from microbial potential in arid soils and promotes diversity and plant health. PLoS ONE, 6(9): e24452, doi: 10.1371/journal.pone.0024452.
18 López-Lozano N E, Heidelberg K B, Nelson W C, et al.2013. Microbial secondary succession in soil microcosms of a desert oasis in the Cuatro Cienegas Basin, Mexico. PeerJ, 1: e47.
19 Margulies M, Egholm M, Altman W E, et al.2005. Genome sequencing in open microfabricated high-density picolitre reactors. Nature, 437(7057): 376-380.
20 McCann C M, Wade M J, Gray N D, et al.2016. Microbial communities in a high arctic polar desert landscape. Frontiers in Microbiology, 7: 419.
21 McKenna P, Hoffmann C, Minkah N, et al.2008. The macaque gut microbiome in health, lentiviral infection, and chronic enterocolitis. PLoS Pathogens, 4(2): e20.
22 Naeem S, Duffy J E, Zavaleta E.2012. The functions of biological diversity in an age of extinction. Science, 336(6087): 1401-1406.
23 Navarro J B, Moser D P, Flores A, et al.2009. Bacterial succession within an ephemeral hypereutrophic Mojave Desert playa Lake. Microbial Ecology, 57(2): 307-320.
24 Neilson J W, Quade J, Ortiz M, et al.2012. Life at the hyperarid margin: novel bacterial diversity in arid soils of the Atacama Desert, Chile. Extremophiles, 16(3): 553-566.
25 Oakley B B, Fiedler T L, Marrazzo J M, et al.2008. Diversity of human vaginal bacterial communities and associations with clinically defined bacterial vaginosis. Applied and Environmental Microbiology, 74(15): 4898-4909.
26 Orlando J, Alfaro M, Bravo L, et al.2010. Bacterial diversity and occurrence of ammonia-oxidizing bacteria in the Atacama Desert soil during a “desert bloom” event. Soil Biology and Biochemistry, 42(7): 1183-1188.
27 Pointing S B, Chan Y, Lacap D C, et al.2009. Highly specialized microbial diversity in hyper-arid polar desert. Proceedings of the National Academy of Sciences of the United States of America, 106(47): 19964-19969.
28 Pollet T, Humbert J F, Tadonléké R D.2014. Planctomycetes in lakes: poor or strong competitors for phosphorus? Applied and Environmental Microbiology, 80(3): 819-828.
29 Pruesse E, Quast C, Knittel K, et al.2007. SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Research, 35(21): 7188-7196.
30 Roesch L F W, Fulthorpe R R, Riva A, et al.2007. Pyrosequencing enumerates and contrasts soil microbial diversity. The ISME Journal, 1(4): 283-290.
31 Rohban R, Amoozegar M A, Ventosa A.2009. Screening and isolation of halophilic bacteria producing extracellular hydrolyses from Howz Soltan Lake, Iran. Journal of Industrial Microbiology & Biotechnology, 36(3): 333-340.
32 Saul-Tcherkas V, Steinberger Y.2011. Soil microbial diversity in the vicinity of a Negev Desert shrub—Reaumuria negevensis. Microbial Ecology, 61(1): 64-81.
33 Shamir I, Steinberger Y.2007. Vertical distribution and activity of soil microbial population in a sandy desert ecosystem. Microbial Ecology, 53(2): 340-347.
34 Skujin? J.1984. Microbial ecology of desert soils. In: Marshall K C. Advances in Microbial Ecology. New York: Springer, 49-91.
35 Sogin M L, Morrison H G, Huber J A, et al.2006. Microbial diversity in the deep sea and the underexplored “rare biosphere”. Proceedings of the National Academy of Sciences of the United States of America, 103(32): 12115-12120.
36 Song W, Kim M, Tripathi B M, et al.2016. Predictable communities of soil bacteria in relation to nutrient concentration and successional stage in a laboratory culture experiment. Environmental Microbiology, 18(6): 1740-1753.
37 Uroz S, Buée M, Murat C, et al.2010. Pyrosequencing reveals a contrasted bacterial diversity between oak rhizosphere and surrounding soil. Environmental Microbiology Reports, 2(2): 281-288.
38 Wang B Z, Zhang C X, Liu J L, et al.2012. Microbial community changes along a land-use gradient of desert soil origin. Pedosphere, 22(5): 593-603.
39 Watanabe K, Nagao N, Toda T, et al.2009. The dominant bacteria shifted from the order “Lactobacillales” to Bacillales and Actinomycetales during a start-up period of large-scale, completely-mixed composting reactor using plastic bottle flakes as bulking agent. World Journal of Microbiology and Biotechnology, 25(5): 803-811.
40 Wessén E, S?derstr?m M, Stenberg M, et al.2011. Spatial distribution of ammonia-oxidizing bacteria and archaea across a 44-hectare farm related to ecosystem functioning. The ISME Journal, 5(7): 1213-1225.
41 Xiong J B, Liu Y Q, Lin X G, et al.2012. Geographic distance and pH drive bacterial distribution in alkaline lake sediments across Tibetan Plateau. Environmental Microbiology, 14(9): 2457-2466.
42 Zeglin L H, Dahm C N, Barrett J E, et al.2011. Bacterial community structure along moisture gradients in the parafluvial sediments of two ephemeral desert streams. Microbial Ecology, 61(3): 543-556.
43 Zhang W, Zhang G S, Liu G X, et al.2012. Bacterial diversity and distribution in the southeast edge of the Tengger Desert and their correlation with soil enzyme activities. Journal of Environmental Sciences, 24(11): 2004-2011.
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