Effects of nitrogen and phosphorus additions on soil microbial community structure and ecological processes in the farmland of Chinese Loess Plateau

doi:10.1007/s40333-023-0023-6

Journal of Arid Land

2023, Vol. 15

Issue (8): 960-974 DOI: 10.1007/s40333-023-0023-6 CSTR: 32276.14.s40333-023-0023-6

Research article

Effects of nitrogen and phosphorus additions on soil microbial community structure and ecological processes in the farmland of Chinese Loess Plateau

KOU Zhaoyang¹, LI Chunyue¹^,^*(

), CHANG Shun¹, MIAO Yu¹, ZHANG Wenting¹, LI Qianxue¹, DANG Tinghui², WANG Yi³

¹School of Geography and Tourism, Shaanxi Normal University, Xi'an 710119, China
²Institute of Soil and Water Conservation, Northwest A&F University, Yangling 712100, China
³State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi'an 710061, China

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Abstract

Microorganisms regulate the responses of terrestrial ecosystems to anthropogenic nutrient inputs. The escalation of anthropogenic activities has resulted in a rise in the primary terrestrial constraining elements, namely nitrogen (N) and phosphorus (P). Nevertheless, the specific mechanisms governing the influence of soil microbial community structure and ecological processes in ecologically vulnerable and delicate semi-arid loess agroecosystems remain inadequately understood. Therefore, we explored the effects of different N and P additions on soil microbial community structure and its associated ecological processes in the farmland of Chinese Loess Plateau based on a 36-a long-term experiment. Nine fertilization treatments with complete interactions of high, medium, and low N and P gradients were set up. Soil physical and chemical properties, along with the microbial community structure were measured in this study. Additionally, relevant ecological processes such as microbial biomass, respiration, N mineralization, and enzyme activity were quantified. To elucidate the relationships between these variables, we examined correlation-mediated processes using statistical techniques, including redundancy analysis (RDA) and structural equation modeling (SEM). The results showed that the addition of N alone had a detrimental effect on soil microbial biomass, mineralized N accumulation, and β-1,4-glucosidase activity. Conversely, the addition of P exhibited an opposing effect, leading to positive influences on these soil parameters. The interactive addition of N and P significantly changed the microbial community structure, increasing microbial activity (microbial biomass and soil respiration), but decreasing the accumulation of mineralized N. Among them, N₂₄P₁₂ treatment showed the greatest increase in the soil nutrient content and respiration. N₁₂P₁₂ treatment increased the overall enzyme activity and total phospholipid fatty acid (PLFA) content by 70.93%. N and P nutrient contents of the soil dominate the microbial community structure and the corresponding changes in hydrolytic enzymes. Soil microbial biomass, respiration, and overall enzyme activity are driven by mineralized N. Our study provides a theoretical basis for exploring energy conversion processes of soil microbial community and environmental sustainability under long-term N and P additions in semi-arid loess areas.

Key words： nitrogen and phosphorus additions microbial community structure farmland ecosystem nitrogen mineralization soil enzyme activity

Received: 30 March 2023 Published: 31 August 2023

Corresponding Authors: * LI Chunyue (E-mail: chunyue_li@snnu.edu.cn)

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	KOU Zhaoyang
	LI Chunyue
	CHANG Shun
	MIAO Yu
	ZHANG Wenting
	LI Qianxue
	DANG Tinghui
	WANG Yi

Cite this article:

KOU Zhaoyang, LI Chunyue, CHANG Shun, MIAO Yu, ZHANG Wenting, LI Qianxue, DANG Tinghui, WANG Yi. Effects of nitrogen and phosphorus additions on soil microbial community structure and ecological processes in the farmland of Chinese Loess Plateau. Journal of Arid Land, 2023, 15(8): 960-974.

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http://jal.xjegi.com/10.1007/s40333-023-0023-6 OR http://jal.xjegi.com/Y2023/V15/I8/960

Table 1 Soil physical-chemical properties under different nitrogen (N) and phosphorus (P) additions

Table 2 Phospholipid fatty acid (PLFA) content under different nitrogen (N) and phosphorus (P) additions

Fig. 1 Soil microbial biomass carbon (MBC; a) and nitrogen (MBN; b) contents under different nitrogen (N) and phosphorus (P) additions. Different lowercase letters among different treatments indicate significant differences at P<0.05 level. Bars are standard errors.

Fig. 2 Soil respiration rate (a), microbial respiration entropy (b), and nitrogen mineralization (c) under different nitrogen (N) and phosphorus (P) additions. Different lowercase letters among different treatments indicate significant differences at P<0.05 level. Bars are standard errors.

Fig. 3 Activities of BG (β-1,4-glucosidase, a), NAG (β-N-acetylglucosaminidase, b), AKP (alkaline phosphatase, c), POD (peroxidase, d), and PPO (polyphenol oxidase, e) under different nitrogen (N) and phosphorus (P) additions. Different lowercase letters among different treatments indicate significant differences at P<0.05 level. Bars are standard errors.

Fig. 4 Redundancy analysis (RDA) of microbial community structure (a) and enzyme activity (b) with soil physical-chemical properties. Red arrows represent soil property variables, and black arrows denote soil microbial taxa (a) and activities of five enzymes (b). TN, total nitrogen; TP, total phosphorus; AP, available phosphorous; SOM, soil organic matter; SMC, soil moisture content; GP, gram-positive bacteria; GN, gram-negative bacteria; GP:GN, gram-positive bacteria:gram-negative bacteria ratio; F:B, fungal:bacterial ratio; BG, β-1,4-glucosidase; POD, peroxidase; AKP, alkaline phosphatase; PPO, polyphenol oxidase; NAG, β-N-acetylglucosaminidas.

Fig. 5 Principal component analysis (PCA) of microbial phospholipid fatty acid (PLFA; a) and soil enzyme activities (b). PC, principal component.

Fig. 6 Structural equation model (SEM) of the effect of nitrogen (N) and phosphorus (P) additions on microbial community structure. Soil microbial community structure in the model was scored by PC1 (principal component). The final model had good fit to the data, with model χ²=5.566, df=6, P=0.474, root mean square error of approximation (RMSEA)=0.000 and comparative fit index (CFI)=1.000. Blue and red arrows indicate positive and negative relationships, width of the arrow is proportional to the positive and negative relationships, and value on the arrow indicates the normalized path coefficient. Asterisks following the values indicate statistical significance of paths, *, P<0.05 level, **, P<0.01 level, ***, P<0.001 level.

Fig. 7 Structural equation model (SEM) of the effects of nitrogen (N) and phosphorus (P) additions on soil ecological processes. Soil enzyme activities in the model were scored by PC1 (principal component). The final model resulted in good fit to the data, with model χ²=3.254, df=6, P=0.776, root mean square error of approximation (RMSEA)=0.000 and comparative fit index (CFI)=1.000. Blue and red arrows indicate positive and negative relationships, respectively. Width of the arrow is proportional to the positive and negative relationships, and value on the arrow indicates the normalized path coefficient. Asterisks following the values indicate statistical significance of paths. *, P<0.05 level, **, P<0.01 level, ***, P<0.001 level.


[1]	Allison S D, Vitousek P M. 2005. Responses of extracellular enzymes to simple and complex nutrient inputs. Soil Biology & Biochemistry, 37(5): 937-944. doi: 10.1016/j.soilbio.2004.09.014

[2]	Baath E, Anderson T H. 2003. Comparison of soil fungal/bacterial ratios in a pH gradient using physiological and PLFA-based techniques. Soil Biology & Biochemistry, 35(7): 955-963. doi: 10.1016/S0038-0717(03)00154-8

[3]	Bao S D. 2000. Soil and Agricultural Chemistry Analysis (3^rd ed.). Beijing: China Agriculture Press, 20-97. (in Chinese)

[4]	Bicharanloo B, Shirvan M B, Keitel C, et al. 2022. Nitrogen and phosphorus availability have stronger effects on gross and net nitrogen mineralisation than wheat rhizodeposition. Geoderma, 405: 115440, doi: 10.1016/j.geoderma.2021.115440. doi: 10.1016/j.geoderma.2021.115440

[5]	Bossio D A, Scow K M, Gunapala N, et al. 1998. Determinants of soil microbial communities: Effects of agricultural management, season, and soil type on phospholipid fatty acid profiles. Microbial Ecology, 36(1): 1-12. pmid: 9622559

[6]	Bunemann E K, Bossio D A, Smithson P C, et al. 2004. Microbial community composition and substrate use in a highly weathered soil as affected by crop rotation and P fertilization. Soil Biology & Biochemistry, 36(6): 889-901. doi: 10.1016/j.soilbio.2004.02.002

[7]	Cai X W, Zhang D, Wang Y Q, et al. 2022. Shift in soil microbial communities along similar to 160 years of natural vegetation restoration on the Loess Plateau of China. Applied Soil Ecology, 173: 104394, doi: 10.1016/j.apsoil.2022.104394. doi: 10.1016/j.apsoil.2022.104394

[8]	Chen J, Luo Y, Li J, et al. 2017. Costimulation of soil glycosidase activity and soil respiration by nitrogen addition. Global Change Biology, 23(3): 1328-1337. doi: 10.1111/gcb.13402 pmid: 27362835

[9]	Chen X, Hao B H, Jing X, et al. 2019. Minor responses of soil microbial biomass, community structure and enzyme activities to nitrogen and phosphorus addition in three grassland ecosystems. Plant and Soil, 444(1-2): 21-37. doi: 10.1007/s11104-019-04250-3

[10]	Cruz A F, Hamel C, Hanson K, et al. 2009. Thirty-seven years of soil nitrogen and phosphorus fertility management shapes the structure and function of the soil microbial community in a brown Chernozem. Plant and Soil, 315(1-2): 173-184. doi: 10.1007/s11104-008-9742-x

[11]	Cui Y X, Fang L C, Guo X B, et al. 2019. Responses of soil bacterial communities, enzyme activities, and nutrients to agricultural-to-natural ecosystem conversion in the Loess Plateau, China. Journal of Soils and Sediments, 19(3): 1427-1440. doi: 10.1007/s11368-018-2110-4

[12]	Dai Z M, Su W Q, Chen H H, et al. 2018. Long-term nitrogen fertilization decreases bacterial diversity and favors the growth of Actinobacteria and Proteobacteria in agro-ecosystems across the globe. Global Change Biology, 24(8): 3452-3461. doi: 10.1111/gcb.14163 pmid: 29645398

[13]	Fanin N, Kardol P, Farrell M, et al. 2019. The ratio of Gram-positive to Gram-negative bacterial PLFA markers as an indicator of carbon availability in organic soils. Soil Biology & Biochemistry, 128: 111-114. doi: 10.1016/j.soilbio.2018.10.010

[14]	Fisk M C, Ratliff T J, Goswami S, et al. 2014. Synergistic soil response to nitrogen plus phosphorus fertilization in hardwood forests. Biogeochemistry, 118(1-3): 195-204. doi: 10.1007/s10533-013-9918-1

[15]	Galloway J N, Townsend A R, Erisman J W, et al. 2008. Transformation of the nitrogen cycle: Recent trends, questions, and potential solutions. Science, 320(5878): 889-892. doi: 10.1126/science.1136674 pmid: 18487183

[16]	Gao W L, Yang H, Kou L, et al. 2015. Effects of nitrogen deposition and fertilization on N transformations in forest soils: A review. Journal of Soils and Sediments, 15(4): 863-879. doi: 10.1007/s11368-015-1064-z

[17]	Grayston S J, Campbell C D, Bardgett R D, et al. 2004. Assessing shifts in microbial community structure across a range of grasslands of differing management intensity using CLPP, PLFA and community DNA techniques. Applied Soil Ecology, 25(1): 63-84. doi: 10.1016/S0929-1393(03)00098-2

[18]	Guo Y, Sun B H, Wang Y, et al. 2017. PLFA fingerprint characteristics of an anthropogenic loess soil under long-term different fertilizations. Scientia Agricultura Sinica, 50(1): 94-103. (in Chinese) doi: 10.3864/j.issn.0578-1752.2017.01.009

[19]	Han J Q, Dong Y Y, Zhang M. 2021. Chemical fertilizer reduction with organic fertilizer effectively improve soil fertility and microbial community from newly cultivated land in the Loess Plateau of China. Applied Soil Ecology, 165: 103966, doi: 10.1016/j.apsoil.2021.103966. doi: 10.1016/j.apsoil.2021.103966

[20]	He D, Xiang X J, He J S, et al. 2016. Composition of the soil fungal community is more sensitive to phosphorus than nitrogen addition in the alpine meadow on the Qinghai-Tibetan Plateau. Biology and Fertility of Soils, 52(8): 1059-1072. doi: 10.1007/s00374-016-1142-4

[21]	He W, Ye W H, Sun M J, et al. 2022. Soil phosphorus availability and stoichiometry determine microbial activity and functional diversity of fluvo-aquic soils under long-term fertilization regimes. Journal of Soils and Sediments, 22(4): 1214-1227. doi: 10.1007/s11368-021-03120-9

[22]	He W Y, Zhang M M, Jin G Z, et al. 2021. Effects of nitrogen deposition on nitrogen-mineralizing enzyme activity and soil microbial community structure in a Korean pine plantation. Microbial Ecology, 81(2): 410-424. doi: 10.1007/s00248-020-01595-6

[23]	Huang J S, Chen W J, Qi K B, et al. 2018. Distinct effects of N and P addition on soil enzyme activities and C distribution in aggregates in a subalpine spruce plantation. Biogeochemistry, 141(2): 199-212. doi: 10.1007/s10533-018-0513-3

[24]	Jing X, Chen X, Fang J Y, et al. 2020. Soil microbial carbon and nutrient constraints are driven more by climate and soil physicochemical properties than by nutrient addition in forest ecosystems. Soil Biology & Biochemistry, 141: 107657, doi: 10.1016/j.soilbio.2019.107657. doi: 10.1016/j.soilbio.2019.107657

[25]	Kaye J P, Hart S C. 1997. Competition for nitrogen between plants and soil microorganisms. Trends in Ecology & Evolution, 12(4): 139-143. doi: 10.1016/S0169-5347(97)01001-X

[26]	Kracmarova M, Kratochvilova H, Uhlik O, et al. 2020. Response of soil microbes and soil enzymatic activity to 20 years of fertilization. Agronomy-Basel, 10(10): 1542, doi: 10.3390/agronomy10101542. doi: 10.3390/agronomy10101542

[27]	Krauss M, Berner A, Perrochet F, et al. 2020. Enhanced soil quality with reduced tillage and solid manures in organic farming-a synthesis of 15 years. Scientific Reports, 10(1): 4403, doi: 10.1038/s41598-020-61320-8. doi: 10.1038/s41598-020-61320-8 pmid: 32157154

[28]	Li J, Li Z A, Wang F M, et al. 2015. Effects of nitrogen and phosphorus addition on soil microbial community in a secondary tropical forest of China. Biology and Fertility of Soils, 51(2): 207-215. doi: 10.1007/s00374-014-0964-1

[29]	Liu J S, Zhang X, Wang H, et al. 2018. Long-term nitrogen fertilization impacts soil fungal and bacterial community structures in a dryland soil of Loess Plateau in China. Journal of Soils and Sediments, 18(4): 1632-1640. doi: 10.1007/s11368-017-1862-6

[30]	Liu Y Z, Zhao C C, Guo J W, et al. 2021. Short-term phosphorus addition augments the effects of nitrogen addition on soil respiration in a typical steppe. Science of the Total Environment, 761: 143211, doi: 10.1016/j.scitotenv.2020.143211. doi: 10.1016/j.scitotenv.2020.143211

[31]	Lu X Y, Lu W, Sun H Y, et al. 2022. Responses of soil respiration to phosphorus addition in global grasslands: A meta-analysis. Journal of Cleaner Production, 349: 131413, doi: 10.1016/j.jclepro.2022.131413. doi: 10.1016/j.jclepro.2022.131413

[32]	Ma S H, Chen G P, Tian D, et al. 2020. Effects of seven-year nitrogen and phosphorus additions on soil microbial community structures and residues in a tropical forest in Hainan Island, China. Geoderma, 361: 114034, doi: 10.1016/j.geoderma.2019.114034. doi: 10.1016/j.geoderma.2019.114034

[33]	Ma X M, Zhou Z, Chen J, et al. 2023. Long-term nitrogen and phosphorus fertilization reveals that phosphorus limitation shapes the microbial community composition and functions in tropical montane forest soil. Science of the Total Environment, 854: 158709, doi: 10.1016/j.scitotenv.2022.158709. doi: 10.1016/j.scitotenv.2022.158709

[34]	Nannipieri P, Trasar-Cepeda C, Dick R P. 2018. Soil enzyme activity: A brief history and biochemistry as a basis for appropriate interpretations and meta-analysis. Biology and Fertility of Soils, 54(1): 11-19. doi: 10.1007/s00374-017-1245-6

[35]	Niu G X, Hasi M, Wang R Z, et al. 2021. Soil microbial community responses to long-term nitrogen addition at different soil depths in a typical steppe. Applied Soil Ecology, 167: 104054, doi: 10.1016/j.apsoil.2021.104054. doi: 10.1016/j.apsoil.2021.104054

[36]	Palansooriya K N, Wong J T F, Hashimoto Y, et al. 2019. Response of microbial communities to biochar-amended soils: A critical review. Biochar, 1(1): 3-22. doi: 10.1007/s42773-019-00009-2

[37]	Saiya-Cork K R, Sinsabaugh R L, Zak D R. 2002. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biology & Biochemistry, 34(9): 1309-1315. doi: 10.1016/S0038-0717(02)00074-3

[38]	Schleuss P, Widdig M, Heintz-Buschart A, et al. 2019. Stoichiometric controls of soil carbon and nitrogen cycling after long-term nitrogen and phosphorus addition in a mesic grassland in South Africa. Soil Biology & Biochemistry, 135: 294-303. doi: 10.1016/j.soilbio.2019.05.018

[39]	Shi Y C, Lalande R, Ziadi N, et al. 2012. An assessment of the soil microbial status after 17 years of tillage and mineral P fertilization management. Applied Soil Ecology, 62: 14-23. doi: 10.1016/j.apsoil.2012.07.004

[40]	Shi Y C, Lalande R, Hamel C, et al. 2013. Seasonal variation of microbial biomass, activity, and community structure in soil under different tillage and phosphorus management practices. Biology and Fertility of Soils, 49(7): 803-818. doi: 10.1007/s00374-013-0773-y

[41]	Siegenthaler M B, Ramoneda J, Frossard E, et al. 2022. Microbial community responses to phosphorus and nitrogen inputs in the organic soil horizons of two contrasting temperate beech forests. Applied Soil Ecology, 172: 104357, doi: 10.1016/j.apsoil.2021.104357. doi: 10.1016/j.apsoil.2021.104357

[42]	Tian D S, Niu S L. 2015. A global analysis of soil acidification caused by nitrogen addition. Environmental Research Letters, 10(2): 024019, doi: 10.1088/1748-9326/10/2/024019. doi: 10.1088/1748-9326/10/2/024019

[43]	Treseder K K. 2008. Nitrogen additions and microbial biomass: A meta-analysis of ecosystem studies. Ecology Letters, 11(10): 1111-1120. doi: 10.1111/j.1461-0248.2008.01230.x pmid: 18673384

[44]	Wang H Y, Nie Y, Butterly C R, et al. 2017. Fertilization alters microbial community composition and functional patterns by changing the chemical nature of soil organic carbon: A field study in a Halosol. Geoderma, 292(15): 17-24. doi: 10.1016/j.geoderma.2017.01.006

[45]	Wang R, Balkanski Y, Boucher O, et al. 2015. Significant contribution of combustion-related emissions to the atmospheric phosphorus budget. Nature Geoscience, 8(1): 48-54. doi: 10.1038/ngeo2324

[46]	Wang R Z, Bicharanloo B, Hou E Q, et al. 2022. Phosphorus supply increases nitrogen transformation rates and retention in soil: A global meta-analysis. Earth's Future, 10(3): e2021EF002479, doi: 10.1029/2021EF002479. doi: 10.1029/2021EF002479

[47]	Wang S Y, Fan T L, Ding N P, et al. 2018. Change characteristics of soil phosphorus and phosphorus fertilizer efficiency in Black Loessial soil of dryland in the Loess Plateau under long-term fertilization. Chinese Journal of Eco-Agriculture, 26(7): 1038-1047. (in Chinese)

[48]	Wang Z R, Yang S, Wang R Z, et al. 2020. Compositional and functional responses of soil microbial communities to long-term nitrogen and phosphorus addition in a calcareous grassland. Pedobiologia, 78: 150612, doi: 10.1016/j.pedobi.2019.150612. doi: 10.1016/j.pedobi.2019.150612

[49]	Widdig M, Heintz-Buschart A, Schleuss P M, et al. 2020. Effects of nitrogen and phosphorus addition on microbial community composition and element cycling in a grassland soil. Soil Biology & Biochemistry, 151: 108041, doi: 10.1016/j.soilbio.2020.108041. doi: 10.1016/j.soilbio.2020.108041

[50]	Wu S H, Huang B H, Gao J, et al. 2019. The effects of afforestation on soil bacterial communities in temperate grassland are modulated by soil chemical properties. Peerj, 7: e6147, doi: 10.7717/peerj.6147. doi: 10.7717/peerj.6147

[51]	Yan W M, Zhong Y Q W, Liu J, et al. 2021. Response of soil respiration to nitrogen fertilization: Evidence from a 6-year field study of croplands. Geoderma, 384: 114829, doi: 10.1016/j.geoderma.2020.114829. doi: 10.1016/j.geoderma.2020.114829

[52]	Zelles L. 1997. Phospholipid fatty acid profiles in selected members of soil microbial communities. Chemosphere, 35(1-2): 275-294. doi: 10.1016/s0045-6535(97)00155-0 pmid: 9232001

[53]	Zeng Q C, Liu D, An S S. 2021. Decoupled diversity patterns in microbial geographic distributions on the arid area (the Loess Plateau). CATENA, 196: 104922, doi: 10.1016/j.catena.2020.104922. doi: 10.1016/j.catena.2020.104922

[54]	Zhang C, Song Z L, Zhuang D H, et al. 2019. Urea fertilization decreases soil bacterial diversity, but improves microbial biomass, respiration, and N-cycling potential in a semiarid grassland. Biology and Fertility of Soils, 55(3): 229-242. doi: 10.1007/s00374-019-01344-z

[55]	Zhang J Y, Ai Z M, Liu H F, et al. 2022. Short-term N addition in a Pinus tabuliformis plantation: Microbial community composition and interactions show different linkages with ecological stoichiometry. Applied Soil Ecology, 174: 104422, doi: 10.1016/j.apsoil.2022.104422. doi: 10.1016/j.apsoil.2022.104422

[56]	Zhang T, Chen H Y, Ruan H H. 2018. Global negative effects of nitrogen deposition on soil microbes. Isme Journal, 12(7): 1817-1825. doi: 10.1038/s41396-018-0096-y pmid: 29588494

[57]	Zhu J X, Wang Q F, He N P, et al. 2016. Imbalanced atmospheric nitrogen and phosphorus depositions in China: Implications for nutrient limitation. Journal of Geophysical Research-Biogeosciences, 121(6): 1605-1616. doi: 10.1002/2016JG003393

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