Rhizobacteria facilitate physiological and biochemical drought tolerance of <i>Halimodendron halodendron</i> (Pall.) Voss

doi:10.1007/s40333-023-0092-6

Journal of Arid Land

2023, Vol. 15

Issue (2): 205-217 DOI: 10.1007/s40333-023-0092-6

Research article

Rhizobacteria facilitate physiological and biochemical drought tolerance of Halimodendron halodendron (Pall.) Voss

Mohammad Hossein TAGHIZADEH¹, Mohammad FARZAM¹^,^*(

), Jafar NABATI²

¹Department of Range and Watershed Management, Ferdowsi University of Mashhad, Mashhad 9178169371, Iran
²Legume Department, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad 9177948974, Iran

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Abstract

Growth-promoting bacteria (GPB) have shown promising effects on serving plants against environmental constraints such as drought. Nevertheless, simultaneous effects of different GPB have less been considered for arid land plants and under field conditions. We investigated the effects of single and combined application of GPB, including free-living nitrogen-fixing bacteria (NFB), phosphate solubilizing bacteria (PSB), potassium solubilizing bacteria (KSB), a combination of NFB, PSB, and KSB (NPK), and control, at three drought stress treatments. In order to better understand the interactions between drought and GPB, we measured the morphological, biochemical, and physiological plant traits. The target plant was salt tree (Halimodendron Halodendron (Pall.) Voss), a legume shrub native to arid lands of Central and West Asia. All biofertilizer treatments enhanced the growth, physiology, and biochemistry of salt tree seedlings, and there were significant differences among the treatments. KSB and PSB treatments increased photosynthetic pigments, but KSB treatment was more efficient in transpiration rate and stomatal regulation and increased the soluble carbohydrates. PSB treatment had the highest effect on root traits, such as taproot length, root volume, cumulative root length, and the ratio of root to shoot. NFB treatment enhanced root diameter and induced biomass translocation between root systems. However, only the application of mixed biofertilizer (i.e., NPK treatment) was the most significant treatment to improve all plant morphological and physiological characteristics of salt tree under drought stress. Therefore, our results provided improvement of some specific plant traits simultaneous with application of three biofertilizers to increase growth and establishment of salt tree seedlings in the degraded arid lands.

Key words： growth-promoting bacteria physiological traits drought stress biofertilizer root traits Halimodendron Halodendron (Pall.) Voss

Received: 13 June 2022 Published: 28 February 2023

Corresponding Authors: ^*Mohammad FARZAM (E-mail: mjankju@um.ac.ir)

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Cite this article:

Mohammad Hossein TAGHIZADEH, Mohammad FARZAM, Jafar NABATI. Rhizobacteria facilitate physiological and biochemical drought tolerance of Halimodendron halodendron (Pall.) Voss. Journal of Arid Land, 2023, 15(2): 205-217.

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http://jal.xjegi.com/10.1007/s40333-023-0092-6 OR http://jal.xjegi.com/Y2023/V15/I2/205

Biofertilizer treatment					Drought level	Biomass and morphological trait
NPK	KSB	PSB	NFB	Control	Drought level	Biomass and morphological trait
41.36±2.10^a	22.44±1.79^g	23.83±1.83^f	31.56±1.33^c	19.72±1.21^h	LD	Total plant height (cm)
34.72±1.04^b	17.12±0.86ⁱ	17.67±1.04ⁱ	26.83±0.63^e	14.11±0.87^j	MD
29.61±0.87^d	13.33±1.49^j	11.61±1.35^k	21.67±1.33^g	7.61±1.38^l	HD
2.140±0.159^a	1.250±0.111^c	0.870±0.140^de	0.924±0.175^d	0.670±0.028^defgh	LD	Stem dry weight (g/Plant)
1.560±0.152^b	0.589±0.112^efghi	0.644±0.026^defgh	0.798±0.007^defg	0.505±0.137^ghi	MD
0.825±0.088^def	0.540±0.127^fghi	0.416±0.061^hi	0.582±0.045^efghi	0.312±0.085ⁱ	HD
2.180±0.212^a	1.580±0.091^b	1.450±0.110 ^b	0.995±0.101^cd	0.837±0.117^de	LD	Leaf dry weight (g/Plant)
1.190±0.112^c	0.572±0.060^fg	0.654±0.063^ef	0.576±0.060^fg	0.383±0.081^g	MD
0.630±0.046^ef	0.474±0.110^fg	0.458±0.098^fg	0.460±0.084^fg	0.364±0.020^g	HD
131.0±15.0^a	119.7±23.0^a	90.3±9.2^b	74.8±5.6^bc	57.1±14.4^cd	LD	Leaf area (cm²/Plant)
80.4±14.8^bc	46.7±5.7^de	56.4±8.4^cd	46.2±7.5^de	27.4±6.1^e	MD
35.4±5.4^de	33.7±6.9^de	33.5±6.5^de	34.8±5.8^de	24.6±1.4^e	HD
44.5±3.4^ab	43.7±2.6^ab	45.4±2.0^a	33.0±0.7^def	37.9±2.0^cde	LD	Taproot length (cm)
45.6±3.4^a	30.9±3.2^f	32.5±0.3^ef	37.5±4.2^cde	30.2±0.6^f	MD
37.9±7.0^cd	36.6±4.8^cde	46.0±7.0^a	39.6±5.2^bc	37.0±4.7^cde	HD
2.180±0.225^a	2.160±0.364^a	1.210±0.101^bc	0.749±0.130^def	0.678±0.101^ef	LD	Root dry weight (g/plant)
1.580±0.198^b	0.730±0.021^def	0.900±0.017^cde	1.110±0.151^cd	0.689±0.019^ef	MD
0.680±0.051^ef	0.364±0.016^f	0.907±0.142^cde	0.912±0.253^cde	0.964±0.055^cde	HD
6.33±0.67^a	5.18±0.82^ab	3.67±0.88^cd	3.00±0.01^def	2.00±0.01^fgh	LD	Root volume (cm³/plant)
4.33±0.33^bc	1.33±0.33^gh	3.63±0.67^cd	3.67±0.33^cd	2.00±0.00^fgh	MD
2.33±0.33^efg	1.00±0.01^h	3.33±0.33^cde	2.42±0.42^efg	2.00±0.58^fgh	HD
95.3±10.7^b	56.4±17.9^c	142.0±12.8^a	41.4±8.3^cde	52.5±18.6^cd	LD	Cumulative root length (m)
58.5±13.2^c	95.0±14.2^b	30.1±5.5^cde	32.5±4.6^cde	42.6±11.7^cde	MD
26.0±4.4^cde	26.1±6.9^cde	47.3±18.6^cde	16.9±0.7^e	21.2±4.7^de	HD
0.504±0.120^cdef	0.956±0.081^ab	0.402±0.149^def	0.776±0.087^bcd	0.797±0.086^abc	LD	Mean root diameter (mm)
0.730±0.244^bcde	0.376±0.102^ef	0.856±0.216^abc	0.783±0.133^abcd	0.331±0.060^f	MD
0.696±0.083^bcdef	0.700±0.157^bcdef	0.669±0.085^bcdef	1.170±0.112^a	1.046±0.125^ab	HD
0.504±0.046^def	0.781±0.163^bcde	0.461±0.096^ef	0.387±0.055^f	0.527±0.066^cdef	LD	Ratio of root to shoot
0.582±0.083^cdef	0.658±0.105^cdef	0.888±0.232^bc	0.814±0.129^bcde	0.700±0.055^bcdef	MD
0.469±0.017^ef	0.362±0.030^f	1.506±0.264^a	0.849±0.146^bcd	1.036±0.155^b	HD

Table 1 Effect of different biofertilizer treatments and drought levels on biomass and morphological traits of salt tree

Table 2 Effect of different biofertilizer treatments and drought levels on photosynthetic traits of salt tree

Fig. 1 Effects of different drought levels and biofertilizer treatments on leaf chlorophyll fluorescence of salt tree. (a), minimum chlorophyll fluorescence; (b), maximum chlorophyll fluorescence; (c), quantum efficiency of photosystem II. Control, without the application of biofertilizer; NFB, free-living nitrogen-fixing bacteria; PSB, phosphate solubilizing bacteria; KSB, potassium solubilizing bacteria; NPK, the combination of NFB, PSB, and KSB; LD, low drought; MD, moderate drought; HD, high drought. Different lowercase letters indicate significantly difference among drought levels and biofertilizer treatments (P≤0.05). Bars are stand errors.

Fig. 2 Effect of different drought levels and biofertilizer treatments on the concentration of photosynthetic pigments of salt tree. (a), chlorophyll a; (b), chlorophyll b; (c), carotenoids; (d), total pigments. Different lowercase letters indicate significantly difference among drought levels and biofertilizer treatments (P≤0.05). Bars are stand errors.

Table 3 Effect of different drought levels and biofertilizer treatments on biochemical traits of salt tree

Table 4 Correlation coefficients between root traits and biochemical traits of salt tree


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