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Journal of Arid Land  2019, Vol. 11 Issue (5): 774-784    DOI: 10.1007/s40333-019-0015-8
Orginal Article     
Effects of water stress and NaCl stress on different life cycle stages of the cold desert annual Lachnoloma lehmannii in China
MAMUT Jannathan1, Dunyan TAN1,2,*(), C BASKIN Carol1,3,4, M BASKIN Jerry1,3
1 Xinjiang Key Laboratory of Grassland Resources and Ecology and Ministry of Education, Key Laboratory for Western Arid Region Grassland Resources and Ecology, College of Grassland and Environment Sciences, Xinjiang Agricultural University, Urumqi 830052, China
2 College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China
3 Department of Biology, University of Kentucky, Lexington, KY 40506, USA
4 Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USA
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For a plant species to complete its life cycle in arid and saline environments, each stage of the life cycle must be tolerant to the harsh environmental conditions. The aim of the study was to determine the effects of water stress (water potentials of -0.05, -0.16, -0.33, -0.56, -0.85 and -1.21 MPa) and NaCl stress (50, 100, 200, 300, 400, 500 and 600 mmol/L NaCl) on seed germination percentage, seedling survival and growth, juvenile growth and plant reproduction of Lachnoloma lehmannii Bunge (Brassicaceae), an cold desert annual that grows in the Junggar Basin of Xinjiang, China in 2010. Results indicated that low water stress (-0.05 and -0.16 MPa) had no significant effect on seed germination percentage. With a decrease in water potential, germination percentage decreased, and no seeds germinated at -0.85 and -1.21 MPa water stresses. Germination percentage of seeds was significantly affected by NaCl stress, and higher germination percentages were observed under non-saline than saline conditions. An increase in NaCl concentrations progressively inhibited seed germination percentage, and no seeds germinated at ≥400 mmol/L NaCl concentration. Non-germinated seeds were transferred from both PEG (polyethylene glycol-6000) and NaCl solutions to distilled water for seed germination recovery. The number of surviving seedlings and their heights and root lengths significantly decreased as NaCl stress increased. About 30% of the plants survived and produced fruits/seeds at 200 mmol/L NaCl concentration. Thus, seed germination, seedling establishment and reproductive stage in the life cycle of L. lehmannii are water- and salt-tolerant, with seedlings being the least tolerant. These tolerances help explain why this species can survive and produce seeds in arid and saline habitats.

Key wordsdrought stress      Lachnoloma lehmannii      salinity tolerance      seed germination      seedling growth     
Published: 10 October 2019
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The first and second authors contributed equally to this work.

Cite this article:

MAMUT Jannathan, Dunyan TAN, C BASKIN Carol, M BASKIN Jerry. Effects of water stress and NaCl stress on different life cycle stages of the cold desert annual Lachnoloma lehmannii in China. Journal of Arid Land, 2019, 11(5): 774-784.

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[1] Ahmad S, Ahmad R, Ashraf M Y, et al.2009. Sunflower (Helianthus annuus L.) response to drought stress at germination and seedling growth stages. Pakistan Journal of Botany, 41(2): 647-654.
[2] Baskin C C, Baskin J M.2014. Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination (2nd ed.). Elsevier: Academic Press, 1-35.
[3] Bhatt A, Bhat N R, Suleiman M K, et al.2018. Effects of storage, mucilage presence, photoperiod, thermoperiod and salinity on germination of Farsetia aegyptia Turra (Brassicaceae) seeds: implications for restoration and seed banks in Arabian Desert. Plant Biosystems, 153(2): 280-287.
[4] Bojović B, Delić G, Topuzović M.2010. Effects of NaCl on seed germination in some species from families Brassicaceae and Solanaceae. Kragujevac Journal of Science, 32: 83-87.
[5] Bradford K J.2002. Applications of hydrothermal time to quantifying and modeling seed germination and dormancy. Weed Science, 50: 248-260.
[6] El-Keblawy A, Al-Rawai A.2005. Effects of salinity, temperature and light on germination of invasive Prosopis juliflora (Sw.) D.C. Journal of Arid Environments, 61(4): 555-565.
[7] Evans C E, Etherington J R.1990. The effects of soil water potential on seed germination of some British plants. New Phytologist, 115(3): 539-548.
[8] Fenner M, Thompson K.2005. The Ecology of Seeds. Cambridge: Cambridge University Press, 331-359.
[9] Flowers T J, Colmer T D.2008. Salinity tolerance in halophytes. New Phytologist, 179(4): 945-963.
[10] Galle A, Haldimann P, Feller U.2007. Photosynthetic performance and water relations in young pubescent oak (Quercus pubescens) trees during drought stress and recovery. New Phytologist, 174(4): 799-810.
[11] Grappin P, Bouinot D, Sotta B, et al.2000. Control of seed dormancy in Nicotiana plumbaginifolia: post-imbibition abscisic acid synthesis imposes dormancy maintenance. Planta, 210(2): 279-285.
[12] Gul B, Weber D J .1999. Effect of salinity, light, and temperature on germination in Allenrolfea occidentalis. Canadian Journal of Botany, 77(2): 240-246.
[13] Gul B, Ansari R, Flowers T J, et al.2013. Germination strategies of halophyte seeds under salinity. Environmental and Experimental Botany, 92: 4-18.
[14] Gutterman Y.1990. Does the germination differ in plants originating in deserts receiving winter or summer rain? Israel Journal of Botany, 39: 355-372. (in Hebrew)
[15] Gutterman Y.1993. Seed Germination in Desert Plants. Berlin: Springer-Verlag, 222-230.
[16] Gutterman Y.2002. Survival Strategies of Annual Desert Plants. Berlin: Springer-Verlag, 211-280.
[17] Haase P, Pugnaire F I, Clark S C, et al.1999. Environmental control of canopy dynamics and photosynthetic rate in the evergreen tussock grass Stipa tenacissima. Plant Ecology, 145(2): 327-339.
[18] Hu X W, Fan Y, Baskin C C, et al.2015. Comparison of the effects of temperature and water potential on seed germination of Fabaceae species from desert and subalpine grassland. American Journal of Botany, 102(5): 649-660.
[19] Huang Z Y, Zhang X S, Zheng G H, et al.2003. Influence of light, temperature, salinity and storage on seed germination of Haloxylon ammodendron. Journal of Arid Environments, 55(3): 453-464.
[20] Khan M A, Rizvi Y.1994. Effect of salinity, temperature, and growth regulators on the germination and early seedling growth of Atriplex griffithii var. stocksii. Canadian Journal of Botany, 72(4): 475-479.
[21] Khan M A, Ungar I A.2001. Effect of germination promoting compounds on the release of primary and salt-enforced seed dormancy in the halophyte Sporobolus arabicus Bioss. Seed Science and Technology, 29: 299-306.
[22] Khan M A, Gulzar S.2003. Germination responses of Sporobolus ioclados: a saline desert grass. Journal of Arid Environments, 53: 387-394.
[23] Khan M A, Gul B.2006. Halophyte seed germination. In: Khan M A, Weber D J. Ecophysiology of High Salinity Tolerant Plants. Netherlands: Springer, 11-30.
[24] Kigel J.1995. Factors affecting germination of arid and semiarid regions. In: Kigel J, Galili G. Seed Development and Germination. New York: Marcel Dekker, 645-700.
[25] Kitajima K, Fenner M .2000. Ecology of seedling regeneration. In: Fenner M. Seeds: the Ecology of Regeneration in Plant Communities (2nd ed.). Wallingford: CABI Publishing, 331-359.
[26] Koller D.1969. The physiology of dormancy and survival of plants in desert environments. Symposium of the Society of Experimental Biology, 23: 449-469.
[27] Li H, Li X, Zhang D, et al.2013. Effects of drought stress on the seed germination and early seedling growth of the endemic desert plant Eremosparton songoricum (Fabaceae). Excli Journal, 12: 89-101.
[28] Ludewig K, Zelle B, Eckstein R L, et al.2014. Differential effects of reduced water potential on the germination of floodplain grassland species indicative of wet and dry habitats. Seed Science Research, 24(1): 49-61.
[29] Ma Y L, Zhang J H, Li X R, et al.2016. Effects of environmental stress on seed germination and seedling growth of Salsola ferganica (Chenopodiaceae). Acta Ecologica Sinica, 36(6): 456-463. (in Chinese)
[30] Mamatriyim N, Yunus Q, Tan D Y.2011. Seed germination and plant growth of four ephemeral species under salt stress. Acta Botanica Boreali-Occidentalia Sinica, 31(8):1618-1627. (in Chinese)
[31] Mamut J, Tan D Y, Baskin C C, et al.2014. Role of trichomes and pericarp in the seed biology of the desert annual Lachnoloma lehmannii (Brassicaceae). Ecological Research, 29(1): 33-44.
[32] Michel B E, Kaufmann M R.1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51(5): 914-916.
[33] Mott J J.1974. Factors affecting seed germination in three annual species from an arid region of Western Australia. Journal of Ecology, 62(3): 699-709.
[34] Qu X X, Huang Z Y, Baskin J M, et al.2008. Effect of temperature, light and salinity on seed germination and radicle growth of the geographically-widespread halophyte shrub Halocnemum strobilaceum. Annals of Botany, 101(2): 293-299.
[35] Raich J W, Rastetter E B, Melillo J M, et al.1991. Potential net primary productivity in south America: application of a global model. Ecological Applications, 1(4): 399-429.
[36] Santo A, Mattana E, Frigau L, et al.2017. Effects of NaCl stress on seed germination and seedling development of Brassica insularis Moris (Brassicaceae). Plant Biology, 19(3): 368-376.
[37] Sokal R R, Rohlf F J .1995. Biometry: the principles and practice of statistics in biological research (3rd ed.). San Francisco: Freeman, 887.
[38] Sun Y, Tan D Y, Baskin C C, et al.2012. Role of mucilage in seed dispersal and germination of the annual ephemeral Alyssum minus (Brassicaceae). Australian Journal of Botany, 60(5): 439-449.
[39] Tang A J, Tian M H, Long C L.2009. Seed dormancy and germination of three herbaceous perennial desert ephemerals from the Junggar Basin, China. Seed Science Research, 19(3): 183-189.
[40] Tevis L.1958. Germination and growth of ephemerals induced by sprinkling a sandy desert. Ecology, 39(4): 681-688.
[41] Tlig T, Gorai M, Neffati M.2008. Germination responses of Diplotaxis harra to temperature and salinity. Flora, 203(5): 421-428.
[42] Ungar I A.1991. Ecophysiology of Vascular Halophytes. Boca Raton: CRC Press, 209.
[43] Ungar I A.1995. Seed germination and seed-bank ecology of halophytes. In: Kigel J, Galili G. Seed Development and Germination. New York: Marcel Dekker, 599-627.
[44] Wang F X, Xu Y G, Wang S, et al.2015. Salinity affects production and salt tolerance of dimorphic seeds of Suaeda salsa. Plant Physiology and Biochemistry, 95: 41-48.
[45] Wei W S, Zhang P, Gao W D, et al.2003. Climate and desert environment evolution in sandstorm source area of Xinjiang, China. Journal of Desert Research, 23(5): 483-487. (in Chinese)
[46] Wetson A M, Cassaniti C, Flowers T J.2008. Do conditions during dormancy influence germination of Suaeda maritma? Annals of Botany, 101(9): 1319-1327.
[47] Xi J B, Zhang F S, Tian C Y.2006. Halophytes in Xinjiang. Beijing: Science Press, 235-255. (in Chinese)
[48] Yang F, Baskin J M, Baskin C C, et al.2017. Divergence in life history traits between two populations of a seed-dimorphic halophyte in response to soil salinity. Frontiers in Plant Science, 8: 1028.
[49] Yang H L, Huang Z Y, Baskin C C, et al.2009. Responses of caryopsis germination, early seedling growth and ramet clonal growth of Bromus inermis to soil salinity. Plant and Soil, 316(1-2): 265-275.
[50] Zehra A, Gul B, Ansari R, et al.2013. Interactive effect of salt, light and temperature on seed germination and recovery of a halophytic grass-Phragmites karka. Pakistan Journal of Botany, 45: 725-736.
[51] Zhang S R, Song J, Wang H, et al.2010. Effect of salinity on seed germination, ion content and photosynthesis of cotyledons in halophytes or xerophyte growing in central Asia. Journal of Plant Ecology, 3(4): 259-267.
[52] Zhang T, Song J, Fan J L, et al.2015. Effects of saline-waterlogging and dryness/moist alternations on seed germination of halophyte and xerophyte. Plant Species Biology, 30(3): 231-236.
[53] Zhou T Y, Lu L L, Yang G, et al.2001. Brassicaceae. In: Wu Z Y, Raven P H. Flora of China 8. Beijing: Science Press, 1-63. (in Chinese)
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