Research article |
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Rhizobacteria facilitate physiological and biochemical drought tolerance of Halimodendron halodendron (Pall.) Voss |
Mohammad Hossein TAGHIZADEH1, Mohammad FARZAM1,*(), Jafar NABATI2 |
1Department of Range and Watershed Management, Ferdowsi University of Mashhad, Mashhad 9178169371, Iran 2Legume Department, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad 9177948974, Iran |
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Abstract Growth-promoting bacteria (GPB) have shown promising effects on serving plants against environmental constraints such as drought. Nevertheless, simultaneous effects of different GPB have less been considered for arid land plants and under field conditions. We investigated the effects of single and combined application of GPB, including free-living nitrogen-fixing bacteria (NFB), phosphate solubilizing bacteria (PSB), potassium solubilizing bacteria (KSB), a combination of NFB, PSB, and KSB (NPK), and control, at three drought stress treatments. In order to better understand the interactions between drought and GPB, we measured the morphological, biochemical, and physiological plant traits. The target plant was salt tree (Halimodendron Halodendron (Pall.) Voss), a legume shrub native to arid lands of Central and West Asia. All biofertilizer treatments enhanced the growth, physiology, and biochemistry of salt tree seedlings, and there were significant differences among the treatments. KSB and PSB treatments increased photosynthetic pigments, but KSB treatment was more efficient in transpiration rate and stomatal regulation and increased the soluble carbohydrates. PSB treatment had the highest effect on root traits, such as taproot length, root volume, cumulative root length, and the ratio of root to shoot. NFB treatment enhanced root diameter and induced biomass translocation between root systems. However, only the application of mixed biofertilizer (i.e., NPK treatment) was the most significant treatment to improve all plant morphological and physiological characteristics of salt tree under drought stress. Therefore, our results provided improvement of some specific plant traits simultaneous with application of three biofertilizers to increase growth and establishment of salt tree seedlings in the degraded arid lands.
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Received: 13 June 2022
Published: 28 February 2023
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Corresponding Authors:
*Mohammad FARZAM (E-mail: mjankju@um.ac.ir)
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[1] |
Abe N, Murata T, Hirota A. 1998. Novel DPPH radical scavengers, bisorbicillinol and demethyltrichodimerol, from a fungus. Bioscience, Biotechnology, and Biochemistry, 62(4): 661-666.
doi: 10.1271/bbb.62.661
pmid: 27392553
|
|
|
[2] |
ALKahtani M D, Attia K A, Hafez Y M, et al. 2020. Chlorophyll fluorescence parameters and antioxidant defense system can display salt tolerance of salt acclimated sweet pepper plants treated with chitosan and plant growth promoting rhizobacteria. Agronomy, 10(8): 1180.
doi: 10.3390/agronomy10081180
|
|
|
[3] |
Arkhipova T, Martynenko E, Sharipova G, et al. 2020. Effects of plant growth promoting rhizobacteria on the content of abscisic acid and salt resistance of wheat plants. Plants, 9(11): 1429.
doi: 10.3390/plants9111429
|
|
|
[4] |
Asif M, Yilmaz O, Ozturk L. 2017. Potassium deficiency impedes elevated carbon dioxide-induced biomass enhancement in well watered or drought-stressed bread wheat. Journal of Plant Nutrition and Soil Science, 180(4): 474-481.
doi: 10.1002/jpln.201600616
|
|
|
[5] |
Attarzadeh M, Balouchi H, Rajaie M, et al. 2019. Improvement of Echinacea purpurea performance by integration of phosphorus with soil microorganisms under different irrigation regimes. Agricultural Water Management, 221: 238-247.
doi: 10.1016/j.agwat.2019.04.022
|
|
|
[6] |
Awad W, Byrne P F, Reid S D, et al. 2018. Great plains winter wheat varies for root length and diameter under drought stress. Agronomy Journal, 110(1): 226-235.
doi: 10.2134/agronj2017.07.0377
|
|
|
[7] |
Ayangbenro A S, Babalola O O. 2021. Reclamation of arid and semi-arid soils: The role of plant growth-promoting archaea and bacteria. Current Plant Biology, 25(1): 100173.
doi: 10.1016/j.cpb.2020.100173
|
|
|
[8] |
Bates L S, Waldren R P, Teare I. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil, 39: 205-207.
doi: 10.1007/BF00018060
|
|
|
[9] |
Chavoshi S, Nourmohamadi G, Madani H, et al. 2018. The effects of biofertilizers on physiological traits and biomass accumulation of red beans (Phaseolus vulgaris cv. Goli) under water stress. Iranian Journal of Plant Physiology, 8(4): 2555-2562.
|
|
|
[10] |
Chiappero J, del Rosario Cappellari L, Alderete L G S, et al. 2019. Plant growth promoting rhizobacteria improve the antioxidant status in Mentha piperita grown under drought stress leading to an enhancement of plant growth and total phenolic content. Industrial Crops and Products, 139(1): 111553.
doi: 10.1016/j.indcrop.2019.111553
|
|
|
[11] |
Dey G, Banerjee P, Sharma R K, et al. 2021. Management of phosphorus in salinity-stressed agriculture for sustainable crop production by salt-tolerant phosphate-solubilizing bacteria—A review. Agronomy, 11(8): 1552.
doi: 10.3390/agronomy11081552
|
|
|
[12] |
Dubois M, Gilles K A, Hamilton J K, et al. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3): 350-356.
doi: 10.1021/ac60111a017
|
|
|
[13] |
Ebrahimi M, Khajehpour M, Naderi A, et al. 2014. Physiological responses of sunflower to water stress under different levels of zinc fertilizer. International Journal of Plant Production, 8(4): 483-504.
|
|
|
[14] |
Ejaz S, Fahad S, Anjum M A, et al. 2020. Role of osmolytes in the mechanisms of antioxidant defense of plants. Sustainable Agriculture Reviews, 39(1): 95-117.
|
|
|
[15] |
Glanz-Idan N, Wolf S. 2020. Upregulation of photosynthesis in mineral nutrition-deficient tomato plants by reduced source-to-sink ratio. Plant Signaling and Behavior, 15(5): 1712543.
doi: 10.1080/15592324.2020.1712543
|
|
|
[16] |
Hassanein A, Ibrahim E, Abou Ali R, et al. 2021. Differential metabolic responses associated with drought tolerance in egyptian rice. Journal of Applied Biology & Biotechnology, 9(4): 37-46.
|
|
|
[17] |
Jha Y. 2017. Potassium mobilizing bacteria: enhance potassium intake in paddy to regulates membrane permeability and accumulate carbohydrates under salinity stress. Brazilian Journal of Biological Sciences, 4(8): 333-344.
doi: 10.21472/bjbs.040812
|
|
|
[18] |
Ju C, Zhang W, Liu Y, et al. 2018. Genetic analysis of seedling root traits reveals the association of root trait with other agronomic traits in maize. BMC Plant Biology, 18(1): 171.
doi: 10.1186/s12870-018-1383-5
pmid: 30111287
|
|
|
[19] |
Kashtoh H, Baek K H. 2021. Structural and functional insights into the role of guard cell ion channels in abiotic stress-induced stomatal closure. Plants, 10(12): 2774.
doi: 10.3390/plants10122774
|
|
|
[20] |
Khan I, Awan S A, Ikram R, et al. 2021. Effects of 24-epibrassinolide on plant growth, antioxidants defense system, and endogenous hormones in two wheat varieties under drought stress. Physiologia Plantarum, 172(2): 696-706.
doi: 10.1111/ppl.13237
|
|
|
[21] |
Khan N, Bano A, Babar M. 2019a. The stimulatory effects of plant growth promoting rhizobacteria and plant growth regulators on wheat physiology grown in sandy soil. Archives of Microbiology, 201(6): 769-785.
doi: 10.1007/s00203-019-01644-w
|
|
|
[22] |
Khan N, Bano A, Babar M A. 2019b. Metabolic and physiological changes induced by plant growth regulators and plant growth promoting rhizobacteria and their impact on drought tolerance in Cicer arietinum L. PLoS ONE, 14(3): e0213040.
doi: 10.1371/journal.pone.0213040
|
|
|
[23] |
Khanghahi M Y, Pirdashti H, Rahimian H, et al. 2019. Leaf photosynthetic characteristics and photosystem II photochemistry of rice (Oryza sativa L.) under potassium-solubilizing bacteria inoculation. Photosynthetica, 57(2): 500-511.
doi: 10.32615/ps.2019.065
|
|
|
[24] |
Kim Y, Chung Y S, Lee E, et al. 2020. Root response to drought stress in rice (Oryza sativa L.). International Journal of Molecular Sciences, 21(4): 1513.
doi: 10.3390/ijms21041513
|
|
|
[25] |
Kumar A, Singh S, Gaurav A K, et al. 2020. Plant growth-promoting bacteria: biological tools for the mitigation of salinity stress in plants. Frontiers in Microbiology, 11(7): 1216.
doi: 10.3389/fmicb.2020.01216
|
|
|
[26] |
Kumar P, Rouphael Y, Cardarelli M, et al. 2017. Vegetable grafting as a tool to improve drought resistance and water use efficiency. Frontiers in Plant Science, 8(1): 1130.
doi: 10.3389/fpls.2017.01130
|
|
|
[27] |
Li T, Ma J, Zou Y, et al. 2020. Quantitative trait loci for seeding root traits and the relationships between root and agronomic traits in common wheat. Genome, 63(1): 27-36.
doi: 10.1139/gen-2019-0116
pmid: 31580743
|
|
|
[28] |
Liu E, Mei X, Yan C, et al. 2016. Effects of water stress on photosynthetic characteristics, dry matter translocation and WUE in two winter wheat genotypes. Agricultural Water Management, 167: 75-85.
doi: 10.1016/j.agwat.2015.12.026
|
|
|
[29] |
Lombardini L, Rossi L. 2019. Ecophysiology of plants in dry environments, in Dryland Ecohydrology. Springer, Netherlands 71-100.
|
|
|
[30] |
Lozano Y M, Aguilar-Trigueros C A, Flaig I C, et al. 2020. Root trait responses to drought are more heterogeneous than leaf trait responses. Functional Ecology, 34(11): 2224-2235.
doi: 10.1111/1365-2435.13656
|
|
|
[31] |
Lynch J P. 2018. Rightsizing root phenotypes for drought resistance. Journal of Experimental Botany, 69(13): 3279-3292.
doi: 10.1093/jxb/ery048
pmid: 29471525
|
|
|
[32] |
Maxwell K, Johnson G N. 2000. Chlorophyll fluorescence—a practical guide. Journal of Experimental Botany, 51(345): 659-668.
doi: 10.1093/jxb/51.345.659
pmid: 10938857
|
|
|
[33] |
Mirzaei M, Ladan Moghadam A, Hakimi L, et al. 2020. Plant growth promoting rhizobacteria (PGPR) improve plant growth, antioxidant capacity, and essential oil properties of lemongrass (Cymbopogon citratus) under water stress. Iranian Journal of Plant Physiology, 10(10): 3155-3166.
|
|
|
[34] |
Mohammadi M H S, Etemadi N, Arab M M, et al. 2017. Molecular and physiological responses of Iranian Perennial ryegrass as affected by Trinexapac ethyl, Paclobutrazol and Abscisic acid under drought stress. Plant Physiology and Biochemistry, 111(1): 129-143.
doi: 10.1016/j.plaphy.2016.11.014
|
|
|
[35] |
Moretti L G, Crusciol C A, Kuramae E E, et al. 2020. Effects of growth-promoting bacteria on soybean root activity, plant development, and yield. Agronomy Journal, 112(1): 418-428.
doi: 10.1002/agj2.20010
|
|
|
[36] |
Rodríguez-Gamir J, Xue J M, Clearwater M J, et al. 2019. Aquaporin regulation in roots controls plant hydraulic conductance, stomatal conductance, and leaf water potential in Pinus radiata under water stress. Plant, Cell & Environment, 42(2): 717-729.
|
|
|
[37] |
Ryan M G, Hubbard R M, Pongracic S, et al. 1996. Foliage, fine-root, woody-tissue and stand respiration in Pinus radiata in relation to nitrogen status. Tree Physiology, 16(3): 333-343.
pmid: 14871734
|
|
|
[38] |
Sati D, Pande V, Pandey S. et al. 2021. Recent advances in PGPR and molecular mechanisms involved in drought stress tolerance. Journal of Soil Science and Plant Nutrition, 1(1):1-9
|
|
|
[39] |
Singleton V L, Rossi J A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3): 144-158.
|
|
|
[40] |
Şükran D, GÜNEŞ T, Sivaci R. 1998. Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany, 22(1): 13-18.
|
|
|
[41] |
Ullah N, Ditta A, Imtiaz M, et al. 2021. Appraisal for organic amendments and plant growth-promoting rhizobacteria to enhance crop productivity under drought stress: A review. Journal of Agronomy and Crop Science, 207(5): 783-802.
doi: 10.1111/jac.12502
|
|
|
[42] |
Ullah S, Khan M Y, Asghar H N, et al. 2017. Differential response of single and co-inoculation of Rhizobium leguminosarum and Mesorhizobium ciceri for inducing water deficit stress tolerance in wheat. Annals of Microbiology, 67: 739-749.
doi: 10.1007/s13213-017-1302-2
|
|
|
[43] |
Vanhees D J, Schneider H M, Sidhu J S, et al. 2022. Soil penetration by maize roots is negatively related to ethylene-induced thickening. Plant, Cell and Environment, 45(3): 789-804.
doi: 10.1111/pce.14175
|
|
|
[44] |
Wang Z, Li G, Sun H, et al. 2018. Effects of drought stress on photosynthesis and photosynthetic electron transport chain in young apple tree leaves. Biology Open, 7(11): bio035279.
|
|
|
[45] |
Xie L, Lehvävirta S, Timonen S, et al. 2018. Species-specific synergistic effects of two plant growth—promoting microbes on green roof plant biomass and photosynthetic efficiency. PLoS ONE, 13(12): e0209432.
doi: 10.1371/journal.pone.0209432
|
|
|
[46] |
Xu X, Du X, Wang F, et al. 2020. Effects of potassium levels on plant growth, accumulation and distribution of carbon, and nitrate metabolism in apple dwarf rootstock seedlings. Frontiers in Plant Science, 11: 904.
doi: 10.3389/fpls.2020.00904
pmid: 32655607
|
|
|
[47] |
Yaghoubi Khanghahi M, Pirdashti H, Rahimian H, et al. 2019. The role of potassium solubilizing bacteria (KSB) inoculations on grain yield, dry matter remobilization and translocation in rice (Oryza sativa L.). Journal of Plant Nutrition, 42(10): 1165-1179.
doi: 10.1080/01904167.2019.1609511
|
|
|
[48] |
Yasin N A, Zaheer M M, Khan W U, et al. 2018. The beneficial role of potassium in Cd-induced stress alleviation and growth improvement in Gladiolus grandiflora L. International Journal of phytoremediation, 20(3): 274-283.
doi: 10.1080/15226514.2017.1374337
|
|
|
[49] |
Yasmin H, Nosheen A, Naz R, et al. 2017. L-tryptophan-assisted PGPR-mediated induction of drought tolerance in maize (Zea mays L.). Journal of Plant Interactions, 12(1): 567-578.
doi: 10.1080/17429145.2017.1402212
|
|
|
[50] |
Yuan Y, Zu M, Sun L, et al. 2022. Isolation and Screening of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase producing PGPR from Paeonia lactiflora rhizosphere and enhancement of plant growth. Scientia Horticulturae, 297: 110956.
doi: 10.1016/j.scienta.2022.110956
|
|
|
[51] |
Zhu Y F, Wu Y X, Hu Y, et al. 2019. Tolerance of two apple rootstocks to short-term salt stress: focus on chlorophyll degradation, photosynthesis, hormone and leaf ultrastructures. Acta Physiologiae Plantarum, 41: 87.
doi: 10.1007/s11738-019-2877-y
|
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