Responses of plant diversity and soil microorganism diversity to nitrogen addition in the desert steppe, China

doi:10.1007/s40333-024-0008-0

Journal of Arid Land

2024, Vol. 16

Issue (3): 447-459 DOI: 10.1007/s40333-024-0008-0 CSTR: 32276.14.s40333-024-0008-0

Research article

Responses of plant diversity and soil microorganism diversity to nitrogen addition in the desert steppe, China

YE He¹^,², HONG Mei¹^,²^,^*(

), XU Xuehui¹^,², LIANG Zhiwei¹^,², JIANG Na¹^,², TU Nare¹^,², WU Zhendan¹^,²

¹College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resources, Hohhot 010018, China
²Key Laboratory of Agricultural Ecological Security and Green Development at University of Inner Mongolia, Hohhot 010018, China

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Abstract

Nitrogen (N) deposition is a significant aspect of global change and poses a threat to terrestrial biodiversity. The impact of plant-soil microbe relationships to N deposition has recently attracted considerable attention. Soil microorganisms have been proven to provide nutrients for specific plant growth, especially in nutrient-poor desert steppe ecosystems. However, the effects of N deposition on plant-soil microbial community interactions in such ecosystems remain poorly understood. To investigate these effects, we conducted a 6-year N-addition field experiment in a Stipa breviflora Griseb. desert steppe in Inner Mongolia Autonomous Region, China. Four N treatment levels (N0, N30, N50, and N100, corresponding to 0, 30, 50, and 100 kg N/(hm²•a), respectively) were applied to simulate atmospheric N deposition. The results showed that N deposition did not significantly affect the aboveground biomass of desert steppe plants. N deposition did not significantly reduce the alfa-diversity of plant and microbial communities in the desert steppe, and low and mediate N additions (N30 and N50) had a promoting effect on them. The variation pattern of plant Shannon index was consistent with that of the soil bacterial Chao1 index. N deposition significantly affected the beta-diversity of plants and soil bacteria, but did not significantly affect fungal communities. In conclusion, N deposition led to co-evolution between desert steppe plants and soil bacterial communities, while fungal communities exhibited strong stability and did not undergo significant changes. These findings help clarify atmospheric N deposition effects on the ecological health and function of the desert steppe.

Key words： soil microorganisms plant-microbial community interaction plant diversity nitrogen deposition desert steppe

Received: 21 November 2023 Published: 31 March 2024

Corresponding Authors: *HONG Mei (E-mail: nmczhm1970@126.com)

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	YE He
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	XU Xuehui
	LIANG Zhiwei
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	TU Nare
	WU Zhendan

Cite this article:

YE He, HONG Mei, XU Xuehui, LIANG Zhiwei, JIANG Na, TU Nare, WU Zhendan. Responses of plant diversity and soil microorganism diversity to nitrogen addition in the desert steppe, China. Journal of Arid Land, 2024, 16(3): 447-459.

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http://jal.xjegi.com/10.1007/s40333-024-0008-0 OR http://jal.xjegi.com/Y2024/V16/I3/447

Table 1 Effects of nitrogen (N) treatments on plant biomass

Fig. 1 Biomass of plant functional group compositions (a), non-metric multidimensional scaling (NMDS) plot illustrating distances between plant community compositions (b), and plant Shannon index (c) under different N treatments. In Figure 1a, R value is the ANOSIM (analysis of similarities) statistic R, and P value is the significance from permutation; in Figure 1b, different lowercase letters within the same treatment indicate significant differences among different plant functional groups at P<0.05 level; in Figure 1c, different lowercase letters indicate significant differences among different N treatments at P<0.05 level. N0, control; N30, 30 kg N/(hm²•a); N50, 50 kg N/(hm²•a); N100, 100 kg N/(hm²•a). Bars are standard errors. The abbreviations are the same in the following figures.

Table 2 Effects of N treatments on soil physical-chemical characteristics

Fig. 2 Relative abundance of dominant bacterial phyla (a), fungal phyla (b), and non-metric multidimensional scaling (NMDS) plot illustrating distances between microbial community composition for bacteria (c) and fungi (d) under different N treatments

Fig. 3 Shannon index and Chao1 richness for soil bacterial community (a and b) and fungal community (c and d) under different N treatments. Different lowercase letters indicate significant differences among different N treatments at P<0.05 level. Bars are standard errors.

Fig. 4 Redundancy analysis (RDA) of environmental factor with bacteria (a) and fungi (b). AGB, aboveground biomass; TN, total nitrogen; SOC, soil organic carbon; C/N, soil organic carbon/total nitrogen.

Table S1 Correlation between environmental factors and dominant bacterial phyla

Table S2 Correlation between environmental factors and dominant fungal phyla

Table S3 Correlation between soil properties and plant community


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