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Journal of Arid Land  2014, Vol. 6 Issue (4): 445-453    DOI: 10.1007/s40333-013-0205-8
Research Articles     
Plantations of native shrub species restore soil microbial diversity in the Horqin Sandy Land, northeastern China
DeMing JIANG, ChengYou CAO, Ying ZHANG, ZhenBo CUI, XiaoShu HAN
1 Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
2 College of Life and Health Sciences, Northeastern University, Shenyang 110004, China
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Abstract  Caragana microphylla Lam., a leguminous shrub species, plays an important role in revegetation in the degraded ecosystems of the Horqin Sandy Land, northeastern China. Large areas planted with this shrub have been artificially established as sand binders for soil protection, which might change the composition of soil bacterial communities with the development of sand dune stabilization. In this paper, we investigated the diversity and composition of native soil bacterial communities in the C. microphylla plantation for sand fixation using polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) to understand the influence of this plantation on sandy soil ecosystem development. We collected soil samples from plantations with an age sequence of 0, 9, 16, and 26 years, as well as from the natural community, to identify the differences among soil bacterial communities. The result showed that bacterial abundance and community composition in the sandy land were affected by the age of the C. microphylla plantation. Moreover, bacterial diversity decreased with increasing plantation age, and the composition of the bacterial community in the 26-year plantation was similar to that in the natural community. Phylogenetic analysis of bands excised from the DGGE gels showed that members of alpha Proteobacterium,
gamma Proteobacterium, Gemmatimonadetes and Chloroflexi were dominant in the sandy land. The stabilization of moving sand dune and development of sand-fixed plantation resulted in an increase of soil fertility, which could drive the structural evolvement of soil bacterial community, and it needs over 20 years for the soil bacterial community to form a stable structure, similar to the case for the natural vegetation.

Key wordsaeolian sand transport      horizontal flux      saltation movement      Sensit      Taklimakan Desert     
Received: 17 May 2013      Published: 12 August 2014

This work was supported by the National Natural Science Foundation of China (40871247) and the China National Twelfth Five-year-plan Key Project (2012BAD16B0302). The authors thank the members of the Wulanaodu Station of Desertification Research, Chinese Academy of Sciences for their technical assistance.

Corresponding Authors: ChengYou CAO     E-mail:
Cite this article:

DeMing JIANG, ChengYou CAO, Ying ZHANG, ZhenBo CUI, XiaoShu HAN. Plantations of native shrub species restore soil microbial diversity in the Horqin Sandy Land, northeastern China. Journal of Arid Land, 2014, 6(4): 445-453.

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Bending G D, Lincoln S D, Sorensen S R, et al. 2003. In-field spatial variability in the degradation of the phenyl-urea herbicide isoproturon is the result of interactions between degradative Sphingomonas spp. and soil pH. Applied and Environmental Microbiology, 69(2): 827–834.

Bowman J P, McCammon S A, Brown M V, et al. 1997. Diversity and association of psychrophilic bacteria in Antarctic Sea ice. Applied and Environmental Microbiology, 63(8): 3068–3078.

Cao C Y, Jiang D M, Teng X H, et al. 2008. Soil chemical and microbiological properties along a chronosequence of Caragana microphylla Lam. plantations in the Horqin Sandy Land of Northeast China. Applied Soil Ecology, 40(1): 78–85.

Cao C Y, Jiang S Y, Zhang Y, et al. 2011. Spatial variability of soil nutrients and microbiological properties after the establishment of leguminous shrub Caragana microphylla Lam. plantation on sand dune in the Horqin Sandy Land of Northeast China. Ecological Engineering, 37(10): 1467–1475.

Crecchio C, Curci M, Pellegrino A, et al. 2007. Soil microbial dynamics and genetic diversity in soil under monoculture wheat grown in different long-term management systems. Soil Biology & Biochemistry, 39(6): 1391–1400.

Drees K P, Neilson J W, Betancourt J L, et al. 2006. Bacterial community structure in the hyperarid core of the Atacama Desert, Chile. Applied and Environmental Microbiology, 72(12): 7902–7908.

Eguchi M, Nishikawa T, Macdonald K, et al. 1996. Responses to stress and nutrient availability by the marine ultramicrobacterium Sphingomonas sp. strain RB2256. Applied and Environmental Microbiology, 62(4): 1287–1294.

Fegatella F, Cavicchioli R. 2000. Physiological responses to starvation in the marine oligotrophic ultramicrobacterium Sphingomonas sp. Strain RB2256. Applied and Environmental Microbiology, 66(5): 2037–2044.

Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4): 783–791.

He J Z, Shen J P, Zhang L M, et al. 2007. Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices. Environmental Microbiology, 9(9): 2364–2374.

Herrera A, Hèry M, James E M, et al. 2007. Species richness and phylogenetic diversity comparisons of soil microbial communities affected by nickel-mining and revegetation efforts in New Caledonia. European Journal of Soil Biology, 43(2): 130–139.

Heuer H, Krsek M, Baker P, et al. 1997. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Applied and Environmental Microbiology, 63(8): 3233–3241.

Hooper D U, Bignell D E, Brown V K, et al. 2000. Interactions between aboveground and belowground biodiversity in terrestrial ecosystems: patterns, mechanisms, and feedbacks. Bioscience, 50(12): 1049–1061.

Hoshino Y T, Matsumoto N. 2007. DNA-versus RNA-based denaturing gradient gel electrophoresis profiles of a bacterial community during replenishment after soil fumigation. Soil Biology & Biochemistry, 39(2): 434–444.

Ibekwe A M, Poss J A, Grattan S R, et al. 2010. Bacterial diversity in cucumber (Cucumis sativus) rhizosphere in response to salinity, soil pH, and boron. Soil Biology & Biochemistry, 42(4): 567–575. Institute of Soil Science, Chinese Academy of Sciences (ISSCAS). 1978. Physical and Chemical Analysis Methods of Soils. Shanghai: Shanghai Science Technology Press, 7–59.

Junier P, Carùc M, Witzel K P. 2009. Effect of common bean (Phaseolus vulgaris L.) on the community composition of ammonia-oxidizing bacteria in soil previously cultivated with Medicago sativa. European Journal of Soil Biology, 45(3): 252–258.

Kardol P, Cornips N J, van Kempen M M L, et al. 2007. Microbe-mediated plant-soil feedback causes historical contingency effects in plant community assembly. Ecological Monographs, 77(2): 147–162.

Kumar S, Tamura K, Nei M. 2004. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics, 5(2): 150–163.

Kuske C R, Ticknor L O, Miller M E, et al. 2002. Comparison of soil bacterial communities in rhizospheres of three plant species and the interspaces in an arid grassland. Applied and Environmental Microbiology, 68(4): 1854–1863.

Liu X M, Zhao H L. 1993. Comprehensive Strategy for Eco-environmental Control in Horqin SandLand. Lanzhou: Gansu Science and Technology Publishing-house, 88–115.

Martensson L, Diez B, Wartiainen I, et al. 2009. Diazotrophic diversity, nifH gene expression and nitrogenase activity in a rice paddy field in Fujian, China. Plant and Soil, 325(1): 207–218.

Medina-Roldán E, Paz-Ferreiro J, Bardgett R D. 2012. Exclusion affects soil and plant communities, but has no impact on soil carbon storage in an upland grassland. Agriculture, Ecosystems and Environment, 149(1): 118–123

Muyzer G, de Waal E C, Uitterlinden A G. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology, 59(3): 695–700.

Nakatsu C H. 2007. Soil microbial community analysis using denaturing gradient gel electrophoresis. Soil Science Society of America Journal, 71(2): 562–571.

Nelson D W, Sommers L E. 1982. Total carbon, organic carbon and organic matter. In: Page A L. Methods of Soil Analysis, Part 2. 2nd ed. Madison: American Society of Agronomy, 539–577.

Nguyen L M, Buttner M P, Cruz P, et al. 2011. Effects of elevated atmospheric CO2 on rhizosphere soil microbial communities in a Mojave Desert ecosystem. Journal of Arid Environments, 75(10): 917–925.

Orlando J, Alfaro M, Bravo L, et al. 2010. Bacterial diversity and occurrence of ammonia-oxidizing bacteria in the Atacama Desert soil during a “desert bloom” event. Soil Biology & Biochemistry, 42(7): 1183–1188.

Prosser J I. 2002. Molecular and functional diversity in soil micro-organisms. Plant and Soil, 244(1): 9–17.

Shen J P, Zhang L M, Guo J F, et al. 2010. Impact of long-term fertilization practices on the abundance and composition of soil bacterial communities in Northeast China. Applied Soil Ecology, 46(1): 119–124.

Smit E, Leefland P, Gommans S, et al. 2001. Diversity and seasonal fluctuations of the dominant members of the bacterial soil community in a wheat field as determined by cultivation and molecular methods. Applied and Environmental Microbiology, 67(5): 2284–2291.

Smith J J, Tow L A, Stafford W, et al. 2006. Bacterial diversity in three different Antarctic cold desert mineral soils. Microbial Ecology, 51(4): 413–421.

Smith N R, Kishchuk B E, Mohan W W. 2008. Effects of wildfire and harvest disturbances on forest soil bacterial communities. Applied and Environmental Microbiology, 74(1): 216–224.

Soule T, Anderson I J, Johnson S L, et al. 2009. Archaeal populations in biological soil crusts from arid lands in North America. Soil Biology & Biochemistry, 41(10): 2069–2074.

Tong X M, Chen F, Yu J, et al. 2008. Phylogenetic identification and microbial diversity in snow of the summit (8201 m) of Cho Oyu Mountain, Tibet. Chinese Science Bulletin, 53(21): 3317–3323.

van der Heijden M G A, Bardgett R D, van Straalen N M. 2008. The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems. Ecology Letters, 11(3): 296–310.

Wardle D A, Bardgett R D, Klironomos J N, et al. 2004. Ecological linkages between aboveground and belowground biota. Science, 304(5677): 1629–1633.

Zak D R, Holmes W E, White D C, et al. 2003. Plant diversity, soil microbial communities, and ecosystem function: are there any links? Ecology, 84(8): 2042–2050.

Zhang T, Zhao H, Li S, et al. 2004. A comparison of different measures for stabilizing moving sand dunes in the Horqin Sandy Land of Inner Mongolia, China. Journal of Arid Environments, 58(2): 202–213.

Zhang W, Hu Y G, Huang G H, et al. 2007. Soil microbial diversity of artificial peashrub plantation on North Loess Plateau of China. Acta Microbiologica Sinica, 47: 751–756.

Zhao H L, Zhou R L, Su Y Z, et al. 2007. Shrub facilitation of desert land restoration in the Horqin Sand Land of Inner Mongolia. Ecological Engineering, 31(1): 1–8.
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